Non-aqueous Fractionation (NAF) for Metabolite Analysis in Subcellular Compartments of
Compartment-specific markers
In-vivo metabolite distribution
Non-aqueous fractionation (NAF)
Journal
Bio-protocol
ISSN: 2331-8325
Titre abrégé: Bio Protoc
Pays: United States
ID NLM: 101635102
Informations de publication
Date de publication:
20 Oct 2019
20 Oct 2019
Historique:
received:
16
07
2019
revised:
17
09
2019
accepted:
28
09
2019
entrez:
3
3
2021
pubmed:
20
10
2019
medline:
20
10
2019
Statut:
epublish
Résumé
The accurate determination of metabolite distribution in subcellular compartments is still challenging in plant science. Various methodologies, such as fluorescence resonance energy transfer-based technology, nuclear magnetic resonance spectroscopy and protoplast fractionation allow the study of metabolite compartmentation. However, large changes in metabolite levels occur during such procedures. Therefore, the non-aqueous fractionation (NAF) technique is currently the best method for the study of
Identifiants
pubmed: 33654900
doi: 10.21769/BioProtoc.3399
pii: e3399
pmc: PMC7853959
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e3399Informations de copyright
Copyright © 2019 The Authors; exclusive licensee Bio-protocol LLC.
Déclaration de conflit d'intérêts
Competing interestsThe authors declare no conflict of interest.
Références
Plant Cell. 2004 Dec;16(12):3304-25
pubmed: 15548738
Planta. 2009 Nov;230(6):1115-27
pubmed: 20183924
Annu Rev Plant Biol. 2013;64:723-46
pubmed: 23330793
Front Plant Sci. 2011 Sep 22;2:55
pubmed: 22645541
J Exp Bot. 2007;58(1):35-47
pubmed: 17030538
Plant Cell Environ. 2009 Apr;32(4):349-67
pubmed: 19143986
Plant Physiol. 2006 Dec;142(4):1574-88
pubmed: 17085515
Methods Mol Biol. 2014;1062:575-96
pubmed: 24057387
Plant Physiol. 2001 Oct;127(2):685-700
pubmed: 11598242
Plant Physiol. 1984 Jul;75(3):542-7
pubmed: 16663663
Planta. 1992 May;187(2):242-6
pubmed: 24178051
Plant Physiol. 1989 Oct;91(2):679-84
pubmed: 16667087
Plant Physiol. 1987 Feb;83(2):399-407
pubmed: 16665257
Hoppe Seylers Z Physiol Chem. 1974 Mar;355(3):378-93
pubmed: 4154892
Mol Cell Proteomics. 2014 Sep;13(9):2246-59
pubmed: 24866124
PLoS One. 2011 Mar 15;6(3):e17806
pubmed: 21423574
Plant J. 2005 Aug;43(4):568-85
pubmed: 16098110
Front Plant Sci. 2016 Dec 22;7:1912
pubmed: 28066469
Elife. 2019 Feb 15;8:
pubmed: 30767893