Herpes Simplex Virus Type 1 Propagation, Titration and Single-step Growth Curves.

Herpes simplex virus type 1 Plaque assay Single-step growth curve Virus propagation

Journal

Bio-protocol
ISSN: 2331-8325
Titre abrégé: Bio Protoc
Pays: United States
ID NLM: 101635102

Informations de publication

Date de publication:
05 Dec 2019
Historique:
received: 24 09 2019
revised: 31 10 2019
accepted: 18 11 2019
entrez: 3 3 2021
pubmed: 5 12 2019
medline: 5 12 2019
Statut: epublish

Résumé

Given the endemic seroprevalence of herpes simplex viruses (HSV), its associated human diseases, and the emergence of acyclovir-resistant strains, there is a continuous need for better antiviral therapies. Towards this aim, identifying mechanistic details of how HSV-1 manipulates infected cells, how it modulates the immune responses, and how it causes diseases are essential. Measuring titers and growth kinetics of clinical isolates and viral mutants are important for a thorough characterization of viral phenotypes

Identifiants

pubmed: 33654936
doi: 10.21769/BioProtoc.3441
pii: e3441
pmc: PMC7853997
doi:

Types de publication

Journal Article

Langues

eng

Pagination

e3441

Informations de copyright

Copyright © 2019 The Authors; exclusive licensee Bio-protocol LLC.

Déclaration de conflit d'intérêts

Competing interestsThe authors declare no competing interests.

Références

APMIS. 1997 Mar;105(3):238-46
pubmed: 9137520
J Gen Virol. 2004 Apr;85(Pt 4):775-786
pubmed: 15039520
Gene Ther. 1996 Oct;3(10):886-91
pubmed: 8908502
Proc Natl Acad Sci U S A. 1998 Jun 9;95(12):7231-5
pubmed: 9618568
Front Cell Infect Microbiol. 2019 Apr 30;9:127
pubmed: 31114761
Adv Virus Res. 1998;51:237-347
pubmed: 9891589
PLoS Pathog. 2010 Jul 08;6(7):e1000991
pubmed: 20628567
J Virol. 2005 Jan;79(2):669-76
pubmed: 15613294
Annu Rev Microbiol. 2013;67:355-74
pubmed: 24024635
Cell Microbiol. 2013 Feb;15(2):248-69
pubmed: 23186167
J Cell Biol. 1997 Mar 10;136(5):1007-21
pubmed: 9060466
J Med Virol. 2008 Mar;80(3):467-77
pubmed: 18205230
PLoS One. 2015 Oct 28;10(10):e0140765
pubmed: 26510007
Curr Probl Pediatr Adolesc Health Care. 2009 Jan;39(1):7-23
pubmed: 19038775
Blood. 2011 Jul 7;118(1):107-15
pubmed: 21562043
Adv Exp Med Biol. 2011;697:221-30
pubmed: 21120729
Bio Protoc. 2014 Aug 5;4(15):
pubmed: 27807592
Pharm Res. 2001 Jan;18(1):90-7
pubmed: 11336359
Rev Med Virol. 2008 Jan-Feb;18(1):35-51
pubmed: 17992661
Traffic. 2006 Feb;7(2):227-37
pubmed: 16420530
Mol Biol Cell. 2002 Aug;13(8):2795-809
pubmed: 12181347
J Virol. 2012 Apr;86(7):3682-700
pubmed: 22258258
J Virol. 2018 Feb 26;92(6):
pubmed: 29298887
Methods Mol Biol. 2014;1144:19-29
pubmed: 24671674
J Virol Methods. 2006 Jul;135(1):118-23
pubmed: 16564579
PLoS Pathog. 2018 Jan 5;14(1):e1006823
pubmed: 29304174
Virol J. 2016 Mar 08;13:38
pubmed: 26952111
J Gen Virol. 2003 Sep;84(Pt 9):2473-2484
pubmed: 12917468
Mol Syst Biol. 2012 Apr 24;8:579
pubmed: 22531119
J Infect Dis. 2002 Oct 15;186 Suppl 1:S3-28
pubmed: 12353183
Lancet. 2001 May 12;357(9267):1513-8
pubmed: 11377626
J Neurovirol. 2013 Aug;19(4):346-50
pubmed: 23775137
Virology. 2007 May 10;361(2):316-24
pubmed: 17223150
Future Virol. 2018 May;13(6):431-443
pubmed: 29967651
Annu Rev Microbiol. 2012;66:153-76
pubmed: 22726218
Virology. 2011 Jan 5;409(1):12-6
pubmed: 21036381
J Virol. 2006 Aug;80(16):8211-24
pubmed: 16873277
Scand J Infect Dis Suppl. 1990;69:19-36
pubmed: 2175939
Gene Ther. 1998 May;5(5):718-22
pubmed: 9797879
Methods Mol Med. 1998;10:1-8
pubmed: 21374218

Auteurs

Linda Grosche (L)

Department of Immune Modulation, Universitätsklinikum Erlangen, Erlangen, Germany.

Katinka Döhner (K)

Institute of Virology, OE5230, Hannover Medical School, Hannover, Germany.

Alexandra Düthorn (A)

Department of Immune Modulation, Universitätsklinikum Erlangen, Erlangen, Germany.

Ana Hickford-Martinez (A)

Institute of Virology, OE5230, Hannover Medical School, Hannover, Germany.

Alexander Steinkasserer (A)

Department of Immune Modulation, Universitätsklinikum Erlangen, Erlangen, Germany.

Beate Sodeik (B)

Institute of Virology, OE5230, Hannover Medical School, Hannover, Germany.

Classifications MeSH