Calvarial Bone Implantation and

Calvaria implantation model Osteoblastic bone metastasis Osteolytic bone metastasis Tumor cell implantation Tumor microenvironment Tumor-bone interaction

Journal

Bio-protocol
ISSN: 2331-8325
Titre abrégé: Bio Protoc
Pays: United States
ID NLM: 101635102

Informations de publication

Date de publication:
05 Feb 2019
Historique:
received: 27 08 2018
revised: 24 12 2018
accepted: 29 01 2019
entrez: 3 3 2021
pubmed: 5 2 2019
medline: 5 2 2019
Statut: epublish

Résumé

Bone is one of common metastasis sites for many types of cancer. In bone metastatic microenvironment, tumor-bone interactions play a significant role in the regulation of osteolytic or osteoblastic bone metastasis. In order to investigate the direct interaction between tumor cells and bone tissue, it is essential to generate appropriate animal models that mimic the behavior of tumor cells in bone metastatic lesions. Calvarial implantation model (bone invasion model) is a newly-established animal model that accurately recapitulates the behavior of tumor cells in the tumor-bone microenvironment. The surgical technique for tumor cell implantation is simpler than intracardiac, intra-arterial, or intraosseous injection techniques. This model can be useful for the identification of key factors driving tumor-induced osteolytic or osteoblastic changes.

Identifiants

pubmed: 33654960
doi: 10.21769/BioProtoc.3151
pii: e3151
pmc: PMC7854057
doi:

Types de publication

Journal Article

Langues

eng

Pagination

e3151

Informations de copyright

Copyright © 2019 The Authors; exclusive licensee Bio-protocol LLC.

Déclaration de conflit d'intérêts

Competing interestsAll authors declare no conflicts of interest.

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Auteurs

Kyoko Hashimoto (K)

Department of Physiology and Cell Biology, Tokyo Medical and Dental University (TMDU), Graduate School, Tokyo 113-8519, Japan.

Shingo Sato (S)

Department of Physiology and Cell Biology, Tokyo Medical and Dental University (TMDU), Graduate School, Tokyo 113-8519, Japan.
Department of Orthopaedic Surgery, Tokyo Medical and Dental University (TMDU), Graduate School, Tokyo 113-8519, Japan.

Hiroki Ochi (H)

Department of Physiology and Cell Biology, Tokyo Medical and Dental University (TMDU), Graduate School, Tokyo 113-8519, Japan.

Shu Takeda (S)

Division of Endocrinology, Toranomon Hospital Endocrine Center, Tokyo 105-8470, Japan.

Mitsuru Futakuchi (M)

Department of Pathology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, 852-8523, Japan.

Classifications MeSH