Immunofluorescence staining of WT-1/Podocalyxin on Mouse kidney sections.
IF
Immunofluorescence
Kidney
Mouse
Podocalyxin
Podocyte
Staining
WT-1
Journal
Bio-protocol
ISSN: 2331-8325
Titre abrégé: Bio Protoc
Pays: United States
ID NLM: 101635102
Informations de publication
Date de publication:
20 Apr 2019
20 Apr 2019
Historique:
received:
18
01
2019
revised:
21
03
2019
accepted:
25
03
2019
entrez:
3
3
2021
pubmed:
20
4
2019
medline:
20
4
2019
Statut:
epublish
Résumé
In glomerular diseases, podocytes are one type of cells involved in dysfunction of glomerular filtration. In these conditions, podocyte proteins expression change. Therefore, immunofluorescent staining of podocytes can be performed to visualize podocyte localization of proteins of interest. In this protocol, we detail a method to stain podocytes with a specific marker WT-1 (
Identifiants
pubmed: 33655004
doi: 10.21769/BioProtoc.3210
pii: e3210
pmc: PMC7854212
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e3210Informations de copyright
Copyright © 2019 The Authors; exclusive licensee Bio-protocol LLC.
Déclaration de conflit d'intérêts
Competing interestsThe authors declare no competing financial interests.
Références
Physiol Rev. 2003 Jan;83(1):253-307
pubmed: 12506131
J Am Soc Nephrol. 2004 Jan;15(1):61-7
pubmed: 14694158
Nat Commun. 2017 Nov 28;8(1):1829
pubmed: 29184126