Time-lapse Whole-field Fluorescence Imaging of Microglia ProcessesMotility in Acute Mouse Hippocampal Slices and Analysis.
Cell Biology
Cell imaging
Cell-based analysis
Hippocampus
Live-cell imaging
Microglia
Mouse
Neuroscience
Physiology
Journal
Bio-protocol
ISSN: 2331-8325
Titre abrégé: Bio Protoc
Pays: United States
ID NLM: 101635102
Informations de publication
Date de publication:
20 Apr 2019
20 Apr 2019
Historique:
received:
20
02
2019
revised:
22
04
2019
accepted:
29
04
2019
entrez:
3
3
2021
pubmed:
20
4
2019
medline:
20
4
2019
Statut:
epublish
Résumé
Microglia are the resident immune cells of the central nervous system (CNS). In the last year, the improvements in the transgenic mouse technologies and imaging techniques have shed light on microglia functions under physiological conditions. Microglia continuously scan the brain parenchyma with their highly motile processes, maintaining tissue homeostasis and participating in neuronal circuits refinement. Here, we describe a protocol that enables us to perform time-lapse imaging of microglial cells in acute hippocampal slices, making image acquisition possible on an electrophysiology rig equipped with a standard imaging system. Using this
Identifiants
pubmed: 33655009
doi: 10.21769/BioProtoc.3220
pii: e3220
pmc: PMC7854105
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e3220Informations de copyright
Copyright © 2019 The Authors; exclusive licensee Bio-protocol LLC.
Déclaration de conflit d'intérêts
Competing interestsThe authors declare no competing interests.
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