Media on-demand: Continuous reconstitution of a chemically defined media directly from solids.


Journal

Biotechnology and bioengineering
ISSN: 1097-0290
Titre abrégé: Biotechnol Bioeng
Pays: United States
ID NLM: 7502021

Informations de publication

Date de publication:
09 2021
Historique:
revised: 26 01 2021
received: 21 12 2020
accepted: 19 02 2021
pubmed: 4 3 2021
medline: 3 3 2022
entrez: 3 3 2021
Statut: ppublish

Résumé

Chemically defined media are reconstituted batchwise and stored in hold tanks until use. To avoid large hold tanks and batchwise production of media, we developed continuous on-demand reconstitutions directly from solids consisting of a hopper and a screw conveyor capable of feeding dry powdered media with the required precision ±5% at low dosing rates of 0.171 g min

Identifiants

pubmed: 33656168
doi: 10.1002/bit.27738
pmc: PMC8451748
doi:

Substances chimiques

Antibodies, Monoclonal 0
Culture Media 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

3382-3394

Informations de copyright

© 2021 The Authors. Biotechnology and Bioengineering published by Wiley Periodicals LLC.

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Auteurs

Daniel Komuczki (D)

Department of Biotechnology, Institute of Bioprocess Science and Engineering, University of Natural Resources and Life Sciences, Vienna, Austria.

Gregory Dutra (G)

Department of Biotechnology, Institute of Bioprocess Science and Engineering, University of Natural Resources and Life Sciences, Vienna, Austria.

Christoph Gstöttner (C)

Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, Netherlands.

Elena Dominguez-Vega (E)

Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, Netherlands.

Alois Jungbauer (A)

Department of Biotechnology, Institute of Bioprocess Science and Engineering, University of Natural Resources and Life Sciences, Vienna, Austria.
Austrian Centre of Industrial Biotechnology, Vienna, Austria.

Peter Satzer (P)

Department of Biotechnology, Institute of Bioprocess Science and Engineering, University of Natural Resources and Life Sciences, Vienna, Austria.
Austrian Centre of Industrial Biotechnology, Vienna, Austria.

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Classifications MeSH