Initial HCV infection of adult hepatocytes triggers a temporally structured transcriptional program containing diverse pro- and anti-viral elements.
Journal
Journal of virology
ISSN: 1098-5514
Titre abrégé: J Virol
Pays: United States
ID NLM: 0113724
Informations de publication
Date de publication:
26 04 2021
26 04 2021
Historique:
entrez:
4
3
2021
pubmed:
5
3
2021
medline:
5
3
2021
Statut:
ppublish
Résumé
Transcriptional profiling provides global snapshots of virus-mediated cellular reprogramming, which can simultaneously encompass pro- and antiviral components. To determine early transcriptional signatures associated with HCV infection of authentic target cells, we performed ex vivo infections of adult primary human hepatocytes (PHHs) from seven donors. Longitudinal sampling identified minimal gene dysregulation at six hours post infection (hpi). In contrast, at 72 hpi, massive increases in the breadth and magnitude of HCV-induced gene dysregulation were apparent, affecting gene classes associated with diverse biological processes. Comparison with HCV-induced transcriptional dysregulation in Huh-7.5 cells identified limited overlap between the two systems. Of note, in PHHs, HCV infection initiated broad upregulation of canonical interferon (IFN)-mediated defense programs, limiting viral RNA replication and abrogating virion release. We further find that constitutive expression of
Identifiants
pubmed: 33658347
pii: JVI.00245-21
doi: 10.1128/JVI.00245-21
pmc: PMC8139656
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
Copyright © 2021 American Society for Microbiology.
Références
Proc Natl Acad Sci U S A. 1995 Jun 6;92(12):5510-4
pubmed: 7539918
Virus Res. 2015 Nov 2;209:100-17
pubmed: 25836277
J Gen Virol. 2020 Jan;101(1):86-95
pubmed: 31821132
Bioinformatics. 2014 Feb 15;30(4):523-30
pubmed: 24336805
Genes Immun. 2015 Sep;16(6):414-21
pubmed: 26066369
Nat Rev Genet. 2009 Jan;10(1):57-63
pubmed: 19015660
Nat Med. 2013 Jul;19(7):879-88
pubmed: 23836238
PLoS One. 2014 Jul 07;9(7):e101386
pubmed: 24999631
J Virol. 2013 Mar;87(5):2935-48
pubmed: 23269812
EMBO Mol Med. 2017 Jun;9(6):816-834
pubmed: 28360091
Nature. 2011 Apr 28;472(7344):481-5
pubmed: 21478870
Emerg Microbes Infect. 2014 Mar;3(3):e21
pubmed: 26038514
Hepatology. 2010 Aug;52(2):443-53
pubmed: 20683944
Nat Rev Cancer. 2010 Apr;10(4):301-9
pubmed: 20332779
Nat Immunol. 2019 Apr;20(4):493-502
pubmed: 30833792
Nat Microbiol. 2019 Jul;4(7):1096-1104
pubmed: 30988429
PLoS Pathog. 2015 Mar 30;11(3):e1004758
pubmed: 25822891
J Virol. 2010 May;84(10):5404-14
pubmed: 20200238
Gastroenterology. 2012 Apr;142(4):978-88
pubmed: 22248663
Nat Rev Gastroenterol Hepatol. 2017 Jun;14(6):343-355
pubmed: 28428634
J Virol. 2002 Dec;76(24):13001-14
pubmed: 12438626
Methods. 2013 Feb;59(2):233-48
pubmed: 23009812
PLoS One. 2011;6(7):e21800
pubmed: 21789182
Proc Natl Acad Sci U S A. 2006 May 9;103(19):7408-13
pubmed: 16651538
Cell Host Microbe. 2017 Aug 9;22(2):176-184
pubmed: 28799903
Viruses. 2012 Apr;4(4):581-612
pubmed: 22590687
Nat Chem Biol. 2016 Dec;12(12):1037-1045
pubmed: 27723751
Trends Microbiol. 2019 Apr;27(4):379-380
pubmed: 30709707
Front Microbiol. 2018 Feb 13;9:207
pubmed: 29487587
Front Immunol. 2019 May 15;10:1019
pubmed: 31156620
Nat Methods. 2008 Jul;5(7):621-8
pubmed: 18516045
Hepatology. 2012 Dec;56(6):2082-93
pubmed: 22711689
Cell Host Microbe. 2009 Dec 17;6(6):513-22
pubmed: 20006840
Methods. 2001 Dec;25(4):402-8
pubmed: 11846609
Sci Adv. 2020 Nov 4;6(45):
pubmed: 33148654
J Immunol. 2016 Oct 15;197(8):3348-3359
pubmed: 27630164
Gut. 2016 Dec;65(12):2017-2028
pubmed: 26276683
J Virol. 2010 Jan;84(2):964-75
pubmed: 19889758
Nature. 2019 Aug;572(7768):199-204
pubmed: 31292543
Hepatology. 2012 Dec;56(6):2060-70
pubmed: 22706965
Nat Methods. 2017 Feb;14(2):135-139
pubmed: 27941783
Cell Host Microbe. 2014 Feb 12;15(2):190-202
pubmed: 24528865
Nature. 2005 Oct 20;437(7062):1167-72
pubmed: 16177806
PLoS Pathog. 2012;8(12):e1003056
pubmed: 23236278
Hepatology. 2011 Dec;54(6):1901-12
pubmed: 22144107
Nat Rev Immunol. 2008 Dec;8(12):911-22
pubmed: 18989317