Potent Neutralization of SARS-CoV-2 by Hetero-bivalent Alpaca Nanobodies Targeting the Spike Receptor-Binding Domain.

Journal

Journal of virology
ISSN: 1098-5514
Titre abrégé: J Virol
Pays: United States
ID NLM: 0113724

Informations de publication

Date de publication:
26 04 2021
Historique:
entrez: 4 3 2021
pubmed: 5 3 2021
medline: 5 3 2021
Statut: ppublish

Résumé

Cell entry by SARS-CoV-2 requires the binding between the receptor-binding domain (RBD) of the viral Spike protein and the cellular angiotensin-converting enzyme 2 (ACE2). As such, RBD has become the major target for vaccine development, while RBD-specific antibodies are pursued as therapeutics. Here, we report the development and characterization of SARS-CoV-2 RBD-specific V

Identifiants

DOI: 10.1128/JVI.02438-20 PMID: 33658349 PMC: PMC8139655
pubmed: 33658349
pii: JVI.02438-20
doi: 10.1128/JVI.02438-20
pmc: PMC8139655
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

Copyright © 2021 Ma et al.

Références

Nature. 2020 Aug;584(7819):120-124
pubmed: 32454512
Nat Commun. 2020 May 4;11(1):2251
pubmed: 32366817
Sci China Life Sci. 2021 Mar;64(3):482-485
pubmed: 32876887
Cell Mol Immunol. 2020 Jul;17(7):773-775
pubmed: 32467617
MAbs. 2018 Jul;10(5):778-795
pubmed: 29733750
Nature. 2020 May;581(7807):221-224
pubmed: 32225175
Nat Rev Drug Discov. 2019 Jul;18(7):485-487
pubmed: 31267082
Nature. 2020 Aug;584(7819):115-119
pubmed: 32454513
Science. 2020 Dec 18;370(6523):1479-1484
pubmed: 33154108
Nat Rev Immunol. 2020 May;20(5):269-270
pubmed: 32273594
Pharmacol Ther. 2017 Jan;169:47-56
pubmed: 27373507
Nature. 2020 Dec;588(7837):205-206
pubmed: 33288887
MAbs. 2020 Jan 1;12(1):1778435
pubmed: 32544372
Cell. 2020 May 14;181(4):894-904.e9
pubmed: 32275855
Clin Infect Dis. 2020 Nov 19;71(16):2027-2034
pubmed: 32221519
Nature. 2020 Mar;579(7798):270-273
pubmed: 32015507
Science. 2020 Mar 13;367(6483):1260-1263
pubmed: 32075877
Lancet. 2020 Feb 22;395(10224):565-574
pubmed: 32007145
N Engl J Med. 2003 May 15;348(20):1953-66
pubmed: 12690092
Nat Struct Mol Biol. 2020 Nov;27(11):1094
pubmed: 33060850
Cell. 2020 Jul 9;182(1):73-84.e16
pubmed: 32425270
Cell. 2020 Apr 16;181(2):281-292.e6
pubmed: 32155444
N Engl J Med. 2012 Nov 8;367(19):1814-20
pubmed: 23075143
BioDrugs. 2020 Feb;34(1):11-26
pubmed: 31686399
Cell Discov. 2020 Sep 29;6:68
pubmed: 33020722
Science. 2020 Aug 21;369(6506):956-963
pubmed: 32540903
Cell Mol Immunol. 2020 Jun;17(6):647-649
pubmed: 32313207
Nat Commun. 2020 Sep 4;11(1):4420
pubmed: 32887876
Euro Surveill. 2021 Jan;26(1):
pubmed: 33413740
Clin Infect Dis. 2020 Nov 5;71(8):1930-1934
pubmed: 32306047
Proc Natl Acad Sci U S A. 2020 Apr 28;117(17):9490-9496
pubmed: 32253318
Science. 2020 Mar 27;367(6485):1444-1448
pubmed: 32132184
PLoS One. 2015 Dec 30;10(12):e0146021
pubmed: 26717316
Aging (Albany NY). 2020 Apr 22;12(8):6536-6542
pubmed: 32320384
Trends Biotechnol. 2014 May;32(5):263-70
pubmed: 24698358
Nat Commun. 2020 Sep 10;11(1):4528
pubmed: 32913273
Cell Host Microbe. 2020 Jun 10;27(6):891-898.e5
pubmed: 32413276
Cell. 2020 Apr 16;181(2):271-280.e8
pubmed: 32142651
Science. 2020 Dec 18;370(6523):1473-1479
pubmed: 33154106
Sci Rep. 2020 Oct 20;10(1):17806
pubmed: 33082473
Nature. 2020 May;581(7807):215-220
pubmed: 32225176
Nat Commun. 2020 Nov 4;11(1):5588
pubmed: 33149112

Auteurs

Huan Ma (H)

Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, China.
Hefei National Laboratory for Physical Sciences at Microscale, Laboratory of Structural Immunology, CAS Key Laboratory of Innate Immunity and Chronic Disease, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, China.

Weihong Zeng (W)

Hefei National Laboratory for Physical Sciences at Microscale, Laboratory of Structural Immunology, CAS Key Laboratory of Innate Immunity and Chronic Disease, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, China.

Xiangzhi Meng (X)

Department of Microbiology, Immunology and Molecular Genetics, University of Texas Health Science Center at San Antonio, San Antonio, Texas, USA.

Xiaoxue Huang (X)

Hefei National Laboratory for Physical Sciences at Microscale, Laboratory of Structural Immunology, CAS Key Laboratory of Innate Immunity and Chronic Disease, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, China.

Yunru Yang (Y)

Hefei National Laboratory for Physical Sciences at Microscale, Laboratory of Structural Immunology, CAS Key Laboratory of Innate Immunity and Chronic Disease, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, China.

Dan Zhao (D)

Hefei National Laboratory for Physical Sciences at Microscale, Laboratory of Structural Immunology, CAS Key Laboratory of Innate Immunity and Chronic Disease, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, China.

Peigen Zhou (P)

Department of Statistics, University of Wisconsin-Madison, Madison, Wisconsin, USA.

Xiaofang Wang (X)

The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, China.

Changcheng Zhao (C)

Department of Infectious Diseases, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, China.

Yong Sun (Y)

Anhui Provincial Center for Disease Control and Prevention, Hefei, Anhui, China.

Peihui Wang (P)

Key Laboratory for Experimental Teratology of Ministry of Education and Advanced Medical Research Institute, Cheeloo College of Medicine, Shandong University, Jinan, China.

Huichao Ou (H)

Hefei National Laboratory for Physical Sciences at Microscale, Laboratory of Structural Immunology, CAS Key Laboratory of Innate Immunity and Chronic Disease, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, China.

Xiaowen Hu (X)

Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, China.

Yan Xiang (Y)

Department of Microbiology, Immunology and Molecular Genetics, University of Texas Health Science Center at San Antonio, San Antonio, Texas, USA jint@ustc.edu.cn xiangy@uthscsa.edu.
ORCID: 0000-0002-7633-1629

Tengchuan Jin (T)

Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, China jint@ustc.edu.cn xiangy@uthscsa.edu.
Hefei National Laboratory for Physical Sciences at Microscale, Laboratory of Structural Immunology, CAS Key Laboratory of Innate Immunity and Chronic Disease, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, China.
CAS Center for Excellence in Molecular Cell Science, Chinese Academy of Science, Shanghai, China.
ORCID: 0000-0002-1395-188X

Classifications MeSH