Cas9-expressing chickens and pigs as resources for genome editing in livestock.
CRISPR-Cas9
Cas9 transgenic chicken
Cas9 transgenic pig
in vivo genome editing
Journal
Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876
Informations de publication
Date de publication:
09 03 2021
09 03 2021
Historique:
entrez:
4
3
2021
pubmed:
5
3
2021
medline:
17
8
2021
Statut:
ppublish
Résumé
Genetically modified animals continue to provide important insights into the molecular basis of health and disease. Research has focused mostly on genetically modified mice, although other species like pigs resemble the human physiology more closely. In addition, cross-species comparisons with phylogenetically distant species such as chickens provide powerful insights into fundamental biological and biomedical processes. One of the most versatile genetic methods applicable across species is CRISPR-Cas9. Here, we report the generation of transgenic chickens and pigs that constitutively express Cas9 in all organs. These animals are healthy and fertile. Functionality of Cas9 was confirmed in both species for a number of different target genes, for a variety of cell types and in vivo by targeted gene disruption in lymphocytes and the developing brain, and by precise excision of a 12.7-kb DNA fragment in the heart. The Cas9 transgenic animals will provide a powerful resource for in vivo genome editing for both agricultural and translational biomedical research, and will facilitate reverse genetics as well as cross-species comparisons.
Identifiants
pubmed: 33658378
pii: 2022562118
doi: 10.1073/pnas.2022562118
pmc: PMC7958376
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
Copyright © 2021 the Author(s). Published by PNAS.
Déclaration de conflit d'intérêts
Competing interest statement: L.C. holds a patent on gene therapy vectors for treating cardiomyopathy that was licensed to DiNAQOR, is a member of the Scientific Advisory Board, and has shares in DiNAQOR.
Références
Gastroenterology. 2012 Nov;143(5):1173-1175.e7
pubmed: 22864254
Mol Cell Biol. 1993 Apr;13(4):2604-13
pubmed: 8455633
Nature. 2006 Jun 8;441(7094):766-9
pubmed: 16760981
Xenotransplantation. 2018 Mar;25(2):e12382
pubmed: 29359453
Sci Rep. 2016 Apr 06;6:23980
pubmed: 27050479
J Vis Exp. 2012 Aug 03;(66):e4017
pubmed: 22895156
PLoS One. 2016 Apr 21;11(4):e0154303
pubmed: 27099923
Transgenic Res. 2016 Oct;25(5):609-16
pubmed: 27034267
Mech Dev. 2006 Jan;123(1):31-41
pubmed: 16325380
J Appl Genet. 2014 Feb;55(1):53-64
pubmed: 24234401
J Am Coll Cardiol. 2010 Jul 27;56(5):414-22
pubmed: 20650363
Proc Natl Acad Sci U S A. 1999 Sep 14;96(19):10806-11
pubmed: 10485907
Sci Rep. 2017 Oct 27;7(1):14246
pubmed: 29079843
Eur J Histochem. 2011 Mar 29;55(1):e4
pubmed: 21556119
Genome Res. 2017 Dec;27(12):2061-2071
pubmed: 29146772
Nat Biotechnol. 2017 Jun;35(6):569-576
pubmed: 28459449
Nucleic Acids Res. 2014 Dec 16;42(22):e168
pubmed: 25300484
Biol Reprod. 2014 Jan 23;90(1):15
pubmed: 24337317
J Virol. 1987 Oct;61(10):3004-12
pubmed: 3041020
Nature. 2014 Oct 16;514(7522):380-4
pubmed: 25119044
Nat Biotechnol. 2016 Jan;34(1):20-2
pubmed: 26641533
PLoS One. 2012;7(10):e43323
pubmed: 23071491
Proc Natl Acad Sci U S A. 2020 Jan 28;117(4):2108-2112
pubmed: 31964810
J Exp Med. 1916 Jul 1;24(1):1-5
pubmed: 19868024
Dev Biol. 2016 Jul 15;415(2):314-325
pubmed: 26777098
BMC Genomics. 2004 Aug 13;5(1):56
pubmed: 15310403
Nat Cell Biol. 1999 Dec;1(8):E203-7
pubmed: 10587659
Nat Protoc. 2015 Oct;10(10):1474-85
pubmed: 26334867
Cold Spring Harb Protoc. 2014 Nov 03;2014(11):1161-6
pubmed: 25368307
Gastroenterology. 2011 Nov;141(5):1762-72
pubmed: 21889923
Eur J Immunol. 2016 Sep;46(9):2137-48
pubmed: 27392810
Macromol Rapid Commun. 2019 Mar;40(5):e1800068
pubmed: 29708298
Am J Cancer Res. 2013 Jun 20;3(3):240-50
pubmed: 23841024
Methods. 2001 May;24(1):43-8
pubmed: 11327801
Mol Reprod Dev. 2008 Jul;75(7):1163-75
pubmed: 18213680
Cell Rep. 2018 Feb 27;22(9):2227-2235
pubmed: 29490262
Nat Med. 2005 Jan;11(1):29-31
pubmed: 15619628
Hum Gene Ther. 2011 Sep;22(9):1143-53
pubmed: 21476867
Nat Med. 2020 Feb;26(2):207-214
pubmed: 31988462
PLoS Pathog. 2017 Feb 23;13(2):e1006206
pubmed: 28231264
Stem Cell Reports. 2015 Dec 8;5(6):1171-1182
pubmed: 26677769
Xenotransplantation. 2020 Jan;27(1):e12560
pubmed: 31591751
Folia Biol (Praha). 2004;50(3-4):107-19
pubmed: 15373344
J Vis Exp. 2007;(9):408
pubmed: 18989448
Vet Immunol Immunopathol. 2008 Aug 15;124(3-4):241-52
pubmed: 18455805
Gen Comp Endocrinol. 2013 Sep 1;190:96-104
pubmed: 23707378
J Immunol. 2014 Apr 1;192(7):3218-27
pubmed: 24567533
J Neurosci Methods. 2014 Aug 15;233:28-33
pubmed: 24906054
Stem Cells Dev. 2016 Nov 15;25(22):1691-1697
pubmed: 27627457
Acc Chem Res. 2019 Jun 18;52(6):1555-1564
pubmed: 31099553
BMC Biotechnol. 2012 Nov 09;12:84
pubmed: 23140586
J Virol. 2014 Mar;88(5):2835-43
pubmed: 24371053
Cell. 2014 Oct 9;159(2):440-55
pubmed: 25263330
Cell Res. 2016 Oct;26(10):1099-1111
pubmed: 27573176
Circulation. 2013 Sep 3;128(10):1066-75
pubmed: 23897866
Cell Res. 2014 Apr;24(4):501-4
pubmed: 24503648
Nature. 2014 Dec 18;516(7531):423-7
pubmed: 25337876
Proc Natl Acad Sci U S A. 2019 Jul 2;116(27):13288-13292
pubmed: 31209054
Xenotransplantation. 2016 Sep;23(5):338-46
pubmed: 27610605
Xenotransplantation. 2009 Nov-Dec;16(6):522-34
pubmed: 20042052
Nat Biotechnol. 2015 Sep;33(9):985-989
pubmed: 26121415
Gene. 2015 Dec 1;573(2):188-97
pubmed: 26358504
PLoS One. 2014 Jul 15;9(7):e102455
pubmed: 25025770
Methods Mol Biol. 2015;1222:37-59
pubmed: 25287337
Transgenic Res. 2014 Feb;23(1):27-38
pubmed: 24065178
Proc Natl Acad Sci U S A. 2013 Dec 10;110(50):20170-5
pubmed: 24282302
Genes Dev. 2015 Jul 15;29(14):1576-85
pubmed: 26178787