Structural insights into membrane remodeling by SNX1.
SNX1
coat complex
cryoelectron microscopy
helical reconstruction
membrane deformation
Journal
Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876
Informations de publication
Date de publication:
09 03 2021
09 03 2021
Historique:
entrez:
4
3
2021
pubmed:
5
3
2021
medline:
17
8
2021
Statut:
ppublish
Résumé
The sorting nexin (SNX) family of proteins deform the membrane to generate transport carriers in endosomal pathways. Here, we elucidate how a prototypic member, SNX1, acts in this process. Performing cryoelectron microscopy, we find that SNX1 assembles into a protein lattice that consists of helical rows of SNX1 dimers wrapped around tubular membranes in a crosslinked fashion. We also visualize the details of this structure, which provides a molecular understanding of how various parts of SNX1 contribute to its ability to deform the membrane. Moreover, we have compared the SNX1 structure with a previously elucidated structure of an endosomal coat complex formed by retromer coupled to a SNX, which reveals how the molecular organization of the SNX in this coat complex is affected by retromer. The comparison also suggests insight into intermediary stages of assembly that results in the formation of the retromer-SNX coat complex on the membrane.
Identifiants
pubmed: 33658379
pii: 2022614118
doi: 10.1073/pnas.2022614118
pmc: PMC7958236
pii:
doi:
Substances chimiques
SNX1 protein, mouse
0
Sorting Nexins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM058615
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM115683
Pays : United States
Organisme : NIGMS NIH HHS
ID : R37 GM058615
Pays : United States
Déclaration de conflit d'intérêts
The authors declare no competing interest.
Références
Cell. 2016 Dec 1;167(6):1623-1635.e14
pubmed: 27889239
Structure. 2020 Apr 7;28(4):393-405.e4
pubmed: 32027819
Cell. 2009 Apr 17;137(2):191-6
pubmed: 19379681
J Comput Chem. 2004 Oct;25(13):1605-12
pubmed: 15264254
Front Aging Neurosci. 2016 Mar 01;8:42
pubmed: 26973516
Nat Cell Biol. 2013 May;15(5):461-71
pubmed: 23563491
Nat Struct Mol Biol. 2016 Oct;23(10):921-932
pubmed: 27595347
Curr Biol. 2004 Oct 26;14(20):1791-800
pubmed: 15498486
J Cell Biol. 2004 Apr;165(1):123-33
pubmed: 15078903
EMBO J. 2012 Nov 28;31(23):4466-80
pubmed: 23085988
PLoS Comput Biol. 2013;9(1):e1002892
pubmed: 23382665
J Cell Biol. 2004 Apr;165(1):111-22
pubmed: 15078902
J Struct Biol. 2007 Jan;157(1):38-46
pubmed: 16859925
J Struct Biol. 2007 Jan;157(1):83-94
pubmed: 16919474
J Struct Biol. 2016 Jan;193(1):1-12
pubmed: 26592709
Cell. 2012 Mar 30;149(1):137-45
pubmed: 22464326
Nat Protoc. 2010 Apr;5(4):725-38
pubmed: 20360767
J Biol Chem. 2010 Jul 23;285(30):23351-8
pubmed: 20484046
Ultramicroscopy. 2000 Dec;85(4):225-34
pubmed: 11125866
Nat Methods. 2015 Jan;12(1):7-8
pubmed: 25549265
Nature. 2018 Sep;561(7724):561-564
pubmed: 30224749
Curr Opin Cell Biol. 2020 Aug;65:17-27
pubmed: 32155566
J Comput Chem. 2005 Dec;26(16):1781-802
pubmed: 16222654
Nat Rev Mol Cell Biol. 2008 Jul;9(7):574-82
pubmed: 18523436
Nat Cell Biol. 2019 Oct;21(10):1219-1233
pubmed: 31576058
Nat Cell Biol. 2011 Jul 03;13(8):914-923
pubmed: 21725319
Cell. 2010 Dec 10;143(6):875-87
pubmed: 21145455
Science. 2004 Jan 23;303(5657):495-9
pubmed: 14645856
J Biol Chem. 2002 Dec 13;277(50):48730-6
pubmed: 12198132
EMBO J. 2007 Nov 14;26(22):4788-800
pubmed: 17948057
Structure. 2021 Jan 7;29(1):61-69.e3
pubmed: 33086035
Nat Commun. 2020 Mar 20;11(1):1506
pubmed: 32198400
Front Aging Neurosci. 2018 Mar 26;10:79
pubmed: 29632483
Cell. 2004 Jan 23;116(2):153-66
pubmed: 14744428
Proc Natl Acad Sci U S A. 2014 Jan 7;111(1):267-72
pubmed: 24344282
Nat Cell Biol. 2011 Jun;13(6):715-21
pubmed: 21602791
Cell. 2014 Feb 27;156(5):882-92
pubmed: 24581490
Acta Crystallogr D Biol Crystallogr. 2004 Dec;60(Pt 12 Pt 1):2126-32
pubmed: 15572765
Acta Crystallogr D Struct Biol. 2019 Oct 1;75(Pt 10):861-877
pubmed: 31588918
Methods. 2016 May 1;100:50-60
pubmed: 26804562
Nat Protoc. 2008;3(12):1941-74
pubmed: 19180078
Cell. 2007 May 18;129(4):761-72
pubmed: 17512409
Nucleic Acids Res. 2015 Jul 1;43(W1):W174-81
pubmed: 25883148
Elife. 2018 Nov 09;7:
pubmed: 30412051
Dev Cell. 2014 Oct 13;31(1):73-86
pubmed: 25284369
Biochem J. 2001 Aug 15;358(Pt 1):7-16
pubmed: 11485546
Cell. 2008 Mar 7;132(5):807-17
pubmed: 18329367
J Cell Biol. 2007 Apr 9;177(1):115-25
pubmed: 17420293
J Chem Theory Comput. 2012 Sep 11;8(9):3257-3273
pubmed: 23341755
Nat Methods. 2017 Apr;14(4):331-332
pubmed: 28250466
Nat Rev Mol Cell Biol. 2018 Nov;19(11):679-696
pubmed: 30194414
Semin Cell Dev Biol. 2010 Jun;21(4):371-80
pubmed: 19914387
Nature. 2007 Oct 25;449(7165):1063-7
pubmed: 17891154
J Cell Biol. 1998 Aug 10;142(3):665-81
pubmed: 9700157