Histopathology for Mohs micrographic surgery with photoacoustic remote sensing microscopy.
Journal
Biomedical optics express
ISSN: 2156-7085
Titre abrégé: Biomed Opt Express
Pays: United States
ID NLM: 101540630
Informations de publication
Date de publication:
01 Jan 2021
01 Jan 2021
Historique:
received:
20
08
2020
revised:
30
10
2020
accepted:
18
11
2020
entrez:
4
3
2021
pubmed:
5
3
2021
medline:
5
3
2021
Statut:
epublish
Résumé
Mohs micrographic surgery (MMS) is a precise oncological technique where layers of tissue are resected and examined with intraoperative histopathology to minimize the removal of normal tissue while completely excising the cancer. To achieve intraoperative pathology, the tissue is frozen, sectioned and stained over a 20- to 60-minute period, then analyzed by the MMS surgeon. Surgery is continued one layer at a time until no cancerous cells remain, meaning MMS can take several hours to complete. Ideally, it would be desirable to circumvent or augment frozen sectioning methods and directly visualize subcellular morphology on the unprocessed excised tissues. Employing photoacoustic remote sensing (PARS) microscopy, we present a non-contact label-free reflection-mode method of performing such visualizations in frozen sections of human skin. PARS leverages endogenous optical absorption contrast within cell nuclei to provide visualizations reminiscent of histochemical staining techniques. Presented here, is the first true one to one comparison between PARS microscopy and standard histopathological imaging in human tissues. We demonstrate the ability of PARS microscopy to provide large grossing scans (>1 cm
Identifiants
pubmed: 33659093
doi: 10.1364/BOE.405869
pii: 405869
pmc: PMC7899511
doi:
Types de publication
Journal Article
Langues
eng
Pagination
654-665Informations de copyright
© 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.
Déclaration de conflit d'intérêts
KB: illumiSonics Inc. (F, I, E, P); DD: illumiSonics Inc. (I); JRM: illumiSonics Inc. (I); PHR: illumiSonics Inc. (F, I, P, S).
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