Preparation of HeLa Total Membranes and Assay of Lipid-inhibition of Serine Palmitoyltransferase Activity.

Ceramide Ceramide synthases Endoplasmic reticulum Homeostasis ORMDL Serine palmitoyltranferase Sphingolipids

Journal

Bio-protocol
ISSN: 2331-8325
Titre abrégé: Bio Protoc
Pays: United States
ID NLM: 101635102

Informations de publication

Date de publication:
20 Jun 2020
Historique:
received: 18 11 2019
revised: 13 04 2020
accepted: 15 04 2020
entrez: 4 3 2021
pubmed: 5 3 2021
medline: 5 3 2021
Statut: epublish

Résumé

Serine palmitoyltranferase (SPT) is a pyridoxal 5' phosphate (PLP)-dependent enzyme that catalyzes the first and rate-limiting step of

Identifiants

pubmed: 33659326
doi: 10.21769/BioProtoc.3656
pii: e3656
pmc: PMC7842383
doi:

Types de publication

Journal Article

Langues

eng

Pagination

e3656

Subventions

Organisme : NHLBI NIH HHS
ID : R01 HL131340
Pays : United States

Informations de copyright

Copyright © 2020 The Authors; exclusive licensee Bio-protocol LLC.

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Auteurs

Muthukumar Kannan (M)

Dept. of Biochemistry and Molecular Biology, Virginia Commonwealth University School of Medicine, Richmond, VA 23298, USA.

Deanna L Davis (DL)

Dept. of Biochemistry and Molecular Biology, Virginia Commonwealth University School of Medicine, Richmond, VA 23298, USA.

John Suemitsu (J)

Dept. of Biochemistry and Molecular Biology, Virginia Commonwealth University School of Medicine, Richmond, VA 23298, USA.

Christopher D Oltorik (CD)

Dept. of Biochemistry and Molecular Biology, Virginia Commonwealth University School of Medicine, Richmond, VA 23298, USA.

Binks Wattenberg (B)

Dept. of Biochemistry and Molecular Biology, Virginia Commonwealth University School of Medicine, Richmond, VA 23298, USA.

Classifications MeSH