Gas Chromatography Detection Protocol of Short-chain Fatty Acids in Mice Feces.
Acidification
Biological samples
Extraction
Gas chromatography
Short-chain fatty acids
Journal
Bio-protocol
ISSN: 2331-8325
Titre abrégé: Bio Protoc
Pays: United States
ID NLM: 101635102
Informations de publication
Date de publication:
05 Jul 2020
05 Jul 2020
Historique:
received:
19
01
2020
revised:
27
03
2020
accepted:
31
03
2020
entrez:
4
3
2021
pubmed:
5
3
2021
medline:
5
3
2021
Statut:
epublish
Résumé
Short-chain fatty acids (SCFAs), which are formed mainly by bacteria fermenting undigested carbohydrates in the colon, they are based on the number of carbon atoms in the carbon chain. Organic fatty acids with less than 6 carbon atoms are called short-chain fatty acids. SCFAs are closely related to various aspects of the human body, so more and more researchers concentrate on SCFAs. This protocol describes, a direct injection gas chromatography detection method with a pretreatment method for extracting SCFA from mice feces by combining acidification. The corresponding sample limit of quantization (LOQ) and limit of detection (LOD) are 0.8-1.0 mg/L and 0.5-0.8 mg/L, respectively. The correlation coefficient of calibration curve is greater than 0.999. The recovery rate of the spiked standard is 80%-102%. This method can be used to analyze and determine SCFAs in mice feces. Therefore, this is an economical, effective and reproducible method for SCFAs measurement in mice samples.
Identifiants
pubmed: 33659342
doi: 10.21769/BioProtoc.3672
pii: e3672
pmc: PMC7842300
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e3672Informations de copyright
Copyright © 2020 The Authors; exclusive licensee Bio-protocol LLC.
Déclaration de conflit d'intérêts
Competing interestsAll authors declare that there are no conflict of interests.
Références
Proc Natl Acad Sci U S A. 2004 Jan 27;101(4):1045-50
pubmed: 14722361
J Chromatogr A. 2005 Sep 23;1088(1-2):49-56
pubmed: 16130732
J Pharm Biomed Anal. 2013 Jun;80:107-15
pubmed: 23542733
Cell Metab. 2015 Dec 1;22(6):954-6
pubmed: 26636491
Methods Mol Biol. 2018;1730:247-256
pubmed: 29363078
J Clin Gastroenterol. 2006 Mar;40(3):235-43
pubmed: 16633129
Pflugers Arch. 2018 Apr;470(4):571-598
pubmed: 29305650
J Chromatogr A. 2000 Jun 9;881(1-2):395-401
pubmed: 10905722
Gastroenterology. 1980 Jun;78(6):1500-7
pubmed: 6768637
Ann Clin Biochem. 2010 Sep;47(Pt 5):447-52
pubmed: 20595408
Nat Rev Gastroenterol Hepatol. 2016 Dec;13(12):691-706
pubmed: 27848961
J Chromatogr A. 2015 Aug 28;1409:282-7
pubmed: 26210111
Med Hypotheses. 2010 Jan;74(1):123-6
pubmed: 19665312
Am J Clin Nutr. 1987 May;45(5 Suppl):1243-55
pubmed: 3034048
Physiol Rev. 2001 Jul;81(3):1031-64
pubmed: 11427691
Biomed Chromatogr. 1991 Sep;5(5):202-6
pubmed: 1742550
Br J Nutr. 2001 Sep;86(3):379-89
pubmed: 11570990
Biomed Chromatogr. 2006 Aug;20(8):674-82
pubmed: 16206138
Food Sci Biotechnol. 2017 Dec 12;27(1):291-298
pubmed: 30263752
Nat Rev Drug Discov. 2002 Apr;1(4):287-99
pubmed: 12120280
Annu Rev Nutr. 2015;35:517-43
pubmed: 26185980
J Chromatogr A. 2011 Aug 26;1218(34):5848-51
pubmed: 21782191
Cell. 2016 Jun 2;165(6):1332-1345
pubmed: 27259147