Nonenzymatic RNA-templated Synthesis of N3'→P5' Phosphoramidate DNA.
3′-amino-2′
3′-dideoxyribonucleotide 5′-phosphoroimidazolide
N3′→P5′ phosphoramidate DNA
Nonenzymatic template-directed synthesis
Origins of life
Journal
Bio-protocol
ISSN: 2331-8325
Titre abrégé: Bio Protoc
Pays: United States
ID NLM: 101635102
Informations de publication
Date de publication:
05 Sep 2020
05 Sep 2020
Historique:
received:
17
03
2020
revised:
10
06
2020
accepted:
15
06
2020
entrez:
4
3
2021
pubmed:
5
3
2021
medline:
5
3
2021
Statut:
epublish
Résumé
The RNA world hypothesis describes a scenario where early life forms relied on RNA to govern both inheritance and catalyze useful chemical reactions. Prior to the emergence of enzymes capable of replicating the RNA genome, a nonenzymatic replication process would have been necessary to initiate Darwinian Evolution. However, the one-pot nonenzymatic RNA chemical copying of templates with mixed-sequences is insufficient to generate strand products long enough to encode useful function. The use of alternate (RNA-like) genetic polymers may overcome hurdles associated with RNA copying, and further our understanding of nonenzymatic copying chemistry. This protocol describes the nonenzymatic copying of RNA templates into N3'→P5' phosphoramidate DNA (3'-NP-DNA). We describe, in detail, the synthesis of 3'-amino-2',3'-dideoxyribonucleotide monomers activated with 2-aminoimidazole (3'-NH
Identifiants
pubmed: 33659395
doi: 10.21769/BioProtoc.3734
pii: e3734
pmc: PMC7842504
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e3734Informations de copyright
Copyright © 2020 The Authors; exclusive licensee Bio-protocol LLC.
Déclaration de conflit d'intérêts
Competing interestsThe authors declare no competing financial interest.
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