Circadian Gene Profiling in Laser Capture Microdissected Mouse Club Cells.
Circadian clock
Club cell
Laser capture micro-dissection
Lung
RNA seq
Journal
Bio-protocol
ISSN: 2331-8325
Titre abrégé: Bio Protoc
Pays: United States
ID NLM: 101635102
Informations de publication
Date de publication:
20 Apr 2020
20 Apr 2020
Historique:
received:
14
10
2019
revised:
27
02
2020
accepted:
22
03
2020
entrez:
4
3
2021
pubmed:
5
3
2021
medline:
5
3
2021
Statut:
epublish
Résumé
Cell heterogeneity is high in tissues like lung. Research conducted on pure population of cells usually offers more insights than bulk tissues, such as circadian clock work. In this protocol, we provide a detailed work flow on how to do circadian clock study by RNA seq in laser capture micro-dissected mouse lung club cells. The method uses frozen tissues and is highly reproduciable.
Identifiants
pubmed: 33659556
doi: 10.21769/BioProtoc.3590
pii: e3590
pmc: PMC7842306
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e3590Informations de copyright
Copyright © 2020 The Authors; exclusive licensee Bio-protocol LLC.
Déclaration de conflit d'intérêts
Competing interestsThe authors don’t have any competing interests.
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