A low-cost computational approach to analyze spiking activity in cockroach sensory neurons.
SpikerBox apparatus
cockroaches
extracellular recordings
spike sorting
Journal
Advances in physiology education
ISSN: 1522-1229
Titre abrégé: Adv Physiol Educ
Pays: United States
ID NLM: 100913944
Informations de publication
Date de publication:
01 Mar 2021
01 Mar 2021
Historique:
entrez:
4
3
2021
pubmed:
5
3
2021
medline:
29
7
2021
Statut:
ppublish
Résumé
Undergraduates use a spike sorting routine developed in Octave to analyze the spiking activity generated from mechanical stimulation of spines of cockroach legs with the inexpensive SpikerBox amplifier and the free software Audacity. Students learn the procedures involved in handling the cockroaches and recording extracellular action potentials (spikes) with the SpikerBox apparatus as well as the importance of spike sorting for analysis in neuroscience. The spike sorting process requires students to choose the spike threshold and spike selection criteria and interact with the clustering process that forms the groups of similar spikes. Once the spike groups are identified, interspike intervals and neuron firing frequencies can be calculated and analyzed. A classic neurophysiology lab exercise is thus adapted to be interdisciplinary for underrepresented students in a small rural college.
Identifiants
pubmed: 33661048
doi: 10.1152/advan.00034.2020
pmc: PMC8091935
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
145-153Subventions
Organisme : NIGMS NIH HHS
ID : P20 GM103451
Pays : United States
Organisme : NIGMS NIH HHS
ID : RL5 GM118969
Pays : United States
Organisme : NIGMS NIH HHS
ID : TL4 GM118971
Pays : United States
Organisme : NIGMS NIH HHS
ID : UL1 GM118970
Pays : United States
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