Stability of the cytosine methylome during post-testicular sperm maturation in mouse.
Journal
PLoS genetics
ISSN: 1553-7404
Titre abrégé: PLoS Genet
Pays: United States
ID NLM: 101239074
Informations de publication
Date de publication:
03 2021
03 2021
Historique:
received:
25
09
2020
accepted:
14
02
2021
revised:
16
03
2021
pubmed:
5
3
2021
medline:
3
8
2021
entrez:
4
3
2021
Statut:
epublish
Résumé
Beyond the haploid genome, mammalian sperm carry a payload of epigenetic information with the potential to modulate offspring phenotypes. Recent studies show that the small RNA repertoire of sperm is remodeled during post-testicular maturation in the epididymis. Epididymal maturation has also been linked to changes in the sperm methylome, suggesting that the epididymis might play a broader role in shaping the sperm epigenome. Here, we characterize the genome-wide methylation landscape in seven germ cell populations from throughout the male reproductive tract. We find very few changes in the cytosine methylation landscape between testicular germ cell populations and cauda epididymal sperm, demonstrating that the sperm methylome is stable throughout post-testicular maturation. Although our sequencing data suggested that caput epididymal sperm exhibit a highly unusual methylome, follow-up studies revealed that this resulted from contamination of caput sperm by extracellular DNA. Extracellular DNA formed web-like structures that ensnared sperm, and was present only in sperm samples obtained from the caput epididymis and vas deferens of virgin males. Curiously, contaminating extracellular DNA was associated with citrullinated histone H3, potentially resulting from a PAD-driven genome decondensation process. Taken together, our data emphasize the stability of cytosine methylation in mammalian sperm, and identify a surprising, albeit transient, period during which sperm are associated with extracellular DNA.
Identifiants
pubmed: 33661909
doi: 10.1371/journal.pgen.1009416
pii: PGENETICS-D-20-01480
pmc: PMC7963034
doi:
Substances chimiques
Cell-Free Nucleic Acids
0
Cytosine
8J337D1HZY
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1009416Subventions
Organisme : NICHD NIH HHS
ID : F31 HD097928
Pays : United States
Organisme : NIEHS NIH HHS
ID : P30 ES013508
Pays : United States
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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