Evaluation of sensitization to the crude extract of Dermatophagoides farinae and its derived allergens, Der f 2 and Zen 1, in dogs with atopic dermatitis in Southern Brazil.
Allergens
/ administration & dosage
Animals
Antigens, Dermatophagoides
/ administration & dosage
Arthropod Proteins
/ administration & dosage
Brazil
Complex Mixtures
/ administration & dosage
Dermatitis, Atopic
/ immunology
Dermatophagoides farinae
/ chemistry
Dog Diseases
/ immunology
Dogs
Female
Hospitals, Animal
Immunization
/ methods
Immunoglobulin E
/ blood
Male
Allergy
House dust mites
Pyroglyphidae
Serology
Journal
Veterinary immunology and immunopathology
ISSN: 1873-2534
Titre abrégé: Vet Immunol Immunopathol
Pays: Netherlands
ID NLM: 8002006
Informations de publication
Date de publication:
Apr 2021
Apr 2021
Historique:
received:
20
06
2019
revised:
22
01
2021
accepted:
28
01
2021
pubmed:
5
3
2021
medline:
18
9
2021
entrez:
4
3
2021
Statut:
ppublish
Résumé
Atopic dermatitis is associated with the production of IgE antibodies against environmental allergens and allergens of the house dust miteDermatophagoides farinae are frequently implicated in the disease. We aimed to observe the allergen-specific IgE against crudeD. farinae, Der f 2 and Zen 1 in dogs with atopic dermatitis and report if these dogs are in contact with material that could shelter mite allergens. 100 dogs with clinical diagnosis of atopic dermatitis were included after exclusion of other forms of pruritic skin disease and dogs that already received specific or non-specific immunotherapy. These dogs were of different breeds and ages and they were presented at a veterinary teaching hospital and a private service of veterinary dermatology, both located in Curitiba, Southern Brazil. At the time of anamnesis, some questions were applied to know the possibility of these dogs having had contact with furniture and textile material which could shelter house dust mites. Sera samples were obtained and further analyzed by ELISA assay to measure serum IgE levels against these allergens with an established cut-off of 0.200 IgE optical density. The allergen-specific IgE positivity against crudeD. farinae (92 %) and Zen 1 (77 %) was higher than Der f 2 (56 %). There was a correlation in sensitization to crude D. farinae and Zen 1 that was not observed between crude D. farinae and Der f 2 and Der f 2 and Zen 1. The sensitization to D. farinae and its allergens was associated with an unrestricted exposition to furniture and textile material. CONCLUSION & CLINICAL RELEVANCE: dogs with atopic dermatitis are frequently sensitized to D. farinae and its allergens, Der f 2 and Zen 1, may be considered major allergens in these dogs. Zen 1 may be the main allergen responsible for the sensitization to crude D. farinae.
Sections du résumé
BACKGROUND
BACKGROUND
Atopic dermatitis is associated with the production of IgE antibodies against environmental allergens and allergens of the house dust miteDermatophagoides farinae are frequently implicated in the disease.
OBJECTIVES
OBJECTIVE
We aimed to observe the allergen-specific IgE against crudeD. farinae, Der f 2 and Zen 1 in dogs with atopic dermatitis and report if these dogs are in contact with material that could shelter mite allergens.
METHODS
METHODS
100 dogs with clinical diagnosis of atopic dermatitis were included after exclusion of other forms of pruritic skin disease and dogs that already received specific or non-specific immunotherapy. These dogs were of different breeds and ages and they were presented at a veterinary teaching hospital and a private service of veterinary dermatology, both located in Curitiba, Southern Brazil. At the time of anamnesis, some questions were applied to know the possibility of these dogs having had contact with furniture and textile material which could shelter house dust mites. Sera samples were obtained and further analyzed by ELISA assay to measure serum IgE levels against these allergens with an established cut-off of 0.200 IgE optical density.
RESULTS
RESULTS
The allergen-specific IgE positivity against crudeD. farinae (92 %) and Zen 1 (77 %) was higher than Der f 2 (56 %). There was a correlation in sensitization to crude D. farinae and Zen 1 that was not observed between crude D. farinae and Der f 2 and Der f 2 and Zen 1. The sensitization to D. farinae and its allergens was associated with an unrestricted exposition to furniture and textile material. CONCLUSION & CLINICAL RELEVANCE: dogs with atopic dermatitis are frequently sensitized to D. farinae and its allergens, Der f 2 and Zen 1, may be considered major allergens in these dogs. Zen 1 may be the main allergen responsible for the sensitization to crude D. farinae.
Identifiants
pubmed: 33662650
pii: S0165-2427(21)00017-9
doi: 10.1016/j.vetimm.2021.110199
pii:
doi:
Substances chimiques
Allergens
0
Antigens, Dermatophagoides
0
Arthropod Proteins
0
Complex Mixtures
0
Dermatophagoides farinae antigen f 2
0
Immunoglobulin E
37341-29-0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
110199Informations de copyright
Copyright © 2021 Elsevier B.V. All rights reserved.