Germline inherited small RNAs facilitate the clearance of untranslated maternal mRNAs in C. elegans embryos.
Animals
Blastomeres
/ cytology
Caenorhabditis elegans
/ embryology
Caenorhabditis elegans Proteins
/ metabolism
Embryo, Nonmammalian
/ cytology
Embryonic Development
/ physiology
Gene Expression Regulation, Developmental
/ genetics
RNA, Messenger, Stored
/ genetics
RNA, Small Untranslated
/ genetics
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
04 03 2021
04 03 2021
Historique:
received:
01
06
2020
accepted:
05
02
2021
entrez:
5
3
2021
pubmed:
6
3
2021
medline:
18
3
2021
Statut:
epublish
Résumé
Inheritance and clearance of maternal mRNAs are two of the most critical events required for animal early embryonic development. However, the mechanisms regulating this process are still largely unknown. Here, we show that together with maternal mRNAs, C. elegans embryos inherit a complementary pool of small non-coding RNAs that facilitate the cleavage and removal of hundreds of maternal mRNAs. These antisense small RNAs are loaded into the maternal catalytically-active Argonaute CSR-1 and cleave complementary mRNAs no longer engaged in translation in somatic blastomeres. Induced depletion of CSR-1 specifically during embryonic development leads to embryonic lethality in a slicer-dependent manner and impairs the degradation of CSR-1 embryonic mRNA targets. Given the conservation of Argonaute catalytic activity, we propose that a similar mechanism operates to clear maternal mRNAs during the maternal-to-zygotic transition across species.
Identifiants
pubmed: 33664268
doi: 10.1038/s41467-021-21691-6
pii: 10.1038/s41467-021-21691-6
pmc: PMC7933186
doi:
Substances chimiques
CSR-1 protein, C elegans
0
Caenorhabditis elegans Proteins
0
RNA, Messenger, Stored
0
RNA, Small Untranslated
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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