Telomeres and replicative cellular aging of the human placenta and chorioamniotic membranes.
Adult
Aging
/ genetics
Cellular Senescence
/ genetics
Chorioallantoic Membrane
/ growth & development
DNA Damage
/ genetics
DNA Replication
/ genetics
Female
Gestational Age
Humans
In Situ Hybridization, Fluorescence
Infant, Newborn
Placenta
/ metabolism
Placentation
Pregnancy
Premature Birth
/ genetics
Telomere
/ genetics
Telomere Homeostasis
/ genetics
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
04 03 2021
04 03 2021
Historique:
received:
24
08
2020
accepted:
16
02
2021
entrez:
5
3
2021
pubmed:
6
3
2021
medline:
15
12
2021
Statut:
epublish
Résumé
Recent hypotheses propose that the human placenta and chorioamniotic membranes (CAMs) experience telomere length (TL)-mediated senescence. These hypotheses are based on mean TL (mTL) measurements, but replicative senescence is triggered by short and dysfunctional telomeres, not mTL. We measured short telomeres by a vanguard method, the Telomere shortest length assay, and telomere-dysfunction-induced DNA damage foci (TIF) in placentas and CAMs between 18-week gestation and at full-term. Both the placenta and CAMs showed a buildup of short telomeres and TIFs, but not shortening of mTL from 18-weeks to full-term. In the placenta, TIFs correlated with short telomeres but not mTL. CAMs of preterm birth pregnancies with intra-amniotic infection showed shorter mTL and increased proportions of short telomeres. We conclude that the placenta and probably the CAMs undergo TL-mediated replicative aging. Further research is warranted whether TL-mediated replicative aging plays a role in all preterm births.
Identifiants
pubmed: 33664422
doi: 10.1038/s41598-021-84728-2
pii: 10.1038/s41598-021-84728-2
pmc: PMC7933277
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
5115Subventions
Organisme : NCI NIH HHS
ID : R01 CA136533
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL116446
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA184572
Pays : United States
Organisme : NICHD NIH HHS
ID : R01 HD071180
Pays : United States
Organisme : NIA NIH HHS
ID : R21 AG067368
Pays : United States
Références
Mol Cell. 2004 May 21;14(4):501-13
pubmed: 15149599
Nat Protoc. 2010 Sep;5(9):1596-607
pubmed: 21085125
Circ Cardiovasc Genet. 2017 Jun;10(3):
pubmed: 28615296
Placenta. 2016 Aug;44:1-5
pubmed: 27452431
Early Hum Dev. 2010 Jul;86(7):451-6
pubmed: 20619976
PLoS One. 2013;8(1):e54013
pubmed: 23326560
EBioMedicine. 2017 Jul;21:14-20
pubmed: 28347656
J Immunol. 2019 Dec 1;203(11):2757-2769
pubmed: 31740550
Am J Reprod Immunol. 2017 May;77(5):
pubmed: 28185343
Nat Commun. 2017 Nov 7;8(1):1356
pubmed: 29116081
Mol Biol Cell. 2004 Aug;15(8):3709-18
pubmed: 15181152
Placenta. 2014 Oct;35(10):791-6
pubmed: 25096951
Nat Cell Biol. 2012 Mar 18;14(4):355-65
pubmed: 22426077
Am J Obstet Gynecol. 2018 Feb;218(2S):S679-S691.e4
pubmed: 29422207
Am J Reprod Immunol. 2014 Apr;71(4):330-58
pubmed: 24417618
Aging Cell. 2015 Jun;14(3):372-81
pubmed: 25684230
J Matern Fetal Neonatal Med. 2015 Sep;28(13):1500-9
pubmed: 25184305
Am J Obstet Gynecol. 2014 Dec;211(6):583-95
pubmed: 25072736
EMBO J. 2019 Dec 2;38(23):e101982
pubmed: 31633821
Nat Commun. 2012 Feb 28;3:708
pubmed: 22426229
Am J Obstet Gynecol. 2017 Mar;216(3):294.e1-294.e8
pubmed: 27865975
Cell. 2001 Oct 5;107(1):67-77
pubmed: 11595186
Am J Obstet Gynecol. 2001 Nov;185(5):1130-6
pubmed: 11717646
Placenta. 2007 Apr;28(4):324-7
pubmed: 16806460
Science. 2006 Mar 3;311(5765):1257
pubmed: 16456035
Biotechniques. 2016 Jun 01;60(6):306-9
pubmed: 27286808
Am J Reprod Immunol. 2018 Nov;80(5):e13049
pubmed: 30225853
Biol Reprod. 2019 May 1;100(5):1290-1305
pubmed: 30590393
Aging Cell. 2008 Aug;7(4):451-8
pubmed: 18462274
Placenta. 2017 Nov;59:61-68
pubmed: 29108638
Aging Cell. 2018 Dec;17(6):e12838
pubmed: 30244523
Am J Obstet Gynecol. 2019 May;220(5):496.e1-496.e8
pubmed: 30690015
Methods Mol Biol. 2017;1534:69-78
pubmed: 27812868
Placenta. 2009 Jun;30(6):539-42
pubmed: 19359039
Semin Hematol. 2013 Apr;50(2):165-74
pubmed: 23956466
Aging Cell. 2014 Oct;13(5):780-6
pubmed: 25040628