Differences in HIV burden in the inflamed and non-inflamed colon from a person living with HIV and ulcerative colitis.

Colon Gut barrier integrity HIV DNA Inflammation Reservoir Ulcerative colitis

Journal

Journal of virus eradication
ISSN: 2055-6640
Titre abrégé: J Virus Erad
Pays: England
ID NLM: 101654142

Informations de publication

Date de publication:
Mar 2021
Historique:
received: 19 11 2020
revised: 10 02 2021
accepted: 12 02 2021
entrez: 5 3 2021
pubmed: 6 3 2021
medline: 6 3 2021
Statut: epublish

Résumé

The greatest obstacle to an HIV cure is the persistence of latently infected cellular reservoirs in people living with HIV (PLWH) taking antiretroviral therapy (ART). However, no consensus exists on the direct link between local tissue inflammation and the HIV burden. Herein, we have compared the levels of local inflammation, epithelial integrity and HIV DNA between inflamed and non-inflamed colon tissue in a PLWH who underwent a colectomy due to ulcerative colitis. We have observed a 27-fold higher frequency of cells harboring HIV DNA in inflamed compared to non-inflamed colon tissue. Analysis of the expression of occludin-1 and claudin-3 confirmed our macroscopic characterization of inflamed and non-inflamed colon. Our results confirm that increased gut permeability and inflammation are associated with a higher frequency of infected cells and suggest that restoring gut barrier integrity may be used as a strategy to reduce inflammation and HIV persistence in the gut.

Identifiants

pubmed: 33664976
doi: 10.1016/j.jve.2021.100033
pii: S2055-6640(21)00006-6
pmc: PMC7906891
doi:

Types de publication

Journal Article

Langues

eng

Pagination

100033

Informations de copyright

© 2021 The Authors.

Déclaration de conflit d'intérêts

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

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Auteurs

Xiaorong Peng (X)

Infectious Diseases and Immunity in Global Health Program, Research Institute, McGill University Health Centre, Montréal, QC, Canada.
Chronic Viral Illness Service, McGill University Health Centre, Montréal, QC, Canada.
State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.

Stéphane Isnard (S)

Infectious Diseases and Immunity in Global Health Program, Research Institute, McGill University Health Centre, Montréal, QC, Canada.
Chronic Viral Illness Service, McGill University Health Centre, Montréal, QC, Canada.
CIHR Canadian HIV Trials Network, Vancouver, BC, Canada.

John Lin (J)

Infectious Diseases and Immunity in Global Health Program, Research Institute, McGill University Health Centre, Montréal, QC, Canada.
Chronic Viral Illness Service, McGill University Health Centre, Montréal, QC, Canada.

Brandon Fombuena (B)

Infectious Diseases and Immunity in Global Health Program, Research Institute, McGill University Health Centre, Montréal, QC, Canada.
Chronic Viral Illness Service, McGill University Health Centre, Montréal, QC, Canada.

Talat Bessissow (T)

Division of Gastroenterology, McGill University Health Center, Montréal, QC, Canada.

Nicolas Chomont (N)

Centre de Recherche du Centre Hospitalier de l'Université de Montréal, Montréal, QC, Canada.

Jean-Pierre Routy (JP)

Infectious Diseases and Immunity in Global Health Program, Research Institute, McGill University Health Centre, Montréal, QC, Canada.
Chronic Viral Illness Service, McGill University Health Centre, Montréal, QC, Canada.
Division of Hematology, McGill University Health Centre, Montréal, QC, Canada.

Classifications MeSH