Diatom extraction: A new technique with heated H2O2. A technical note.

Diatoms extraction Drowning Forensic pathology Forensic veterinary Heated hydrogen peroxide method

Journal

Legal medicine (Tokyo, Japan)
ISSN: 1873-4162
Titre abrégé: Leg Med (Tokyo)
Pays: Ireland
ID NLM: 100889186

Informations de publication

Date de publication:
May 2021
Historique:
received: 12 06 2020
revised: 29 01 2021
accepted: 07 02 2021
pubmed: 6 3 2021
medline: 9 11 2021
entrez: 5 3 2021
Statut: ppublish

Résumé

The best method of diatom identification in animal and human tissues is still an important discussion topic, in terms of effectiveness and reliability. In this technical note, authors propose a new method of extraction of diatoms using heated hydrogen peroxide from animal and human tissue samples. This method has been compared with the traditional method of digestion with acids. The results of the comparison show that heated hydrogen peroxide extraction is more efficient in terms of reduction of sediment, extraction of the material and preservation of diatoms proving to be a viable alternative to conventional approaches with acids in terms of costs and operator safety.

Identifiants

pubmed: 33667932
pii: S1344-6223(21)00025-0
doi: 10.1016/j.legalmed.2021.101861
pii:
doi:

Substances chimiques

Hydrogen Peroxide BBX060AN9V

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

101861

Informations de copyright

Copyright © 2021 Elsevier B.V. All rights reserved.

Auteurs

Francesca Marezza (F)

Institute of Legal Medicine, Department of Medicine and Surgery, University of Parma, Italy. Electronic address: francesca.marezza@icloud.com.

Cristina Marchetti (C)

Department of Veterinary Science, University of Parma, Italy.

Giovanni Lanzi (G)

Institute of Legal Medicine, Department of Medicine and Surgery, University of Parma, Italy.

Andrea Lusetti (A)

Institute of Legal Medicine, Department of Medicine and Surgery, University of Parma, Italy.

Alessia Bertani (A)

Institute of Legal Medicine, Department of Medicine and Surgery, University of Parma, Italy.

Rossana Cecchi (R)

Institute of Legal Medicine, Department of Medicine and Surgery, University of Parma, Italy.

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Classifications MeSH