Patients with Proliferative Lupus Nephritis Have Autoantibodies That React to Moesin and Demonstrate Increased Glomerular Moesin Expression.
autoantibodies
glomerulonephritis
lupus nephritis
systemic lupus erythematosus
target antigens
Journal
Journal of clinical medicine
ISSN: 2077-0383
Titre abrégé: J Clin Med
Pays: Switzerland
ID NLM: 101606588
Informations de publication
Date de publication:
16 Feb 2021
16 Feb 2021
Historique:
received:
04
01
2021
revised:
28
01
2021
accepted:
08
02
2021
entrez:
6
3
2021
pubmed:
7
3
2021
medline:
7
3
2021
Statut:
epublish
Résumé
Kidney involvement in systemic lupus erythematosus (SLE)-termed lupus nephritis (LN)-is a severe manifestation of SLE that can lead to end-stage kidney disease (ESKD). LN is characterized by immune complex deposition and inflammation in the glomerulus. We tested the hypothesis that autoantibodies targeting podocyte and glomerular cell proteins contribute to the development of immune complex formation in LN. We used Western blotting with SLE sera from patients with and without LN to identify target antigens in human glomerular and cultured human-derived podocyte membrane proteins. Using liquid chromatography-tandem mass spectrometry (LC-MS/MS), we identified the proteins in the gel regions corresponding to reactive bands observed with sera from LN patients. We identified 102 proteins that were present in both the podocyte and glomerular samples. We identified 10 high-probability candidates, including moesin, using bioinformatic analysis. Confirmation of moesin as a target antigen was conducted using immunohistochemical analysis (IHC) of kidney biopsy tissue and enzyme-linked immunosorbent assay (ELISA) to detect circulating antibodies. By IHC, biopsies from patients with proliferative lupus nephritis (PLN, class III/IV) demonstrated significantly increased glomerular expression of moesin (
Identifiants
pubmed: 33669337
pii: jcm10040793
doi: 10.3390/jcm10040793
pmc: PMC7920286
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : NIH HHS
ID : R01AI024717
Pays : United States
Organisme : NIAID NIH HHS
ID : U01 AI130830
Pays : United States
Organisme : NIH HHS
ID : K08DK102542
Pays : United States
Organisme : U.S. Department of Veterans Affairs
ID : I01BX001834
Organisme : NIH HHS
ID : U01AI130830
Pays : United States
Organisme : NIDDK NIH HHS
ID : K08 DK102542
Pays : United States
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