Role of Shear Stress and tPA Concentration in the Fibrinolytic Potential of Thrombi.
fibrinolysis
plasminogen activator inhibitor-1
shear
thrombi
tissue plasminogen activator
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
20 Feb 2021
20 Feb 2021
Historique:
received:
01
02
2021
revised:
11
02
2021
accepted:
13
02
2021
entrez:
6
3
2021
pubmed:
7
3
2021
medline:
15
4
2021
Statut:
epublish
Résumé
The resolution of arterial thrombi is critically dependent on the endogenous fibrinolytic system. Using well-established and complementary whole blood models, we investigated the endogenous fibrinolytic potential of the tissue-type plasminogen activator (tPA) and the intra-thrombus distribution of fibrinolytic proteins, formed ex vivo under shear. tPA was present at physiologically relevant concentrations and fibrinolysis was monitored using an FITC-labelled fibrinogen tracer. Thrombi were formed from anticoagulated blood using a Chandler Loop and from non-anticoagulated blood perfused over specially-prepared porcine aorta strips under low (212 s
Identifiants
pubmed: 33672724
pii: ijms22042115
doi: 10.3390/ijms22042115
pmc: PMC7924342
pii:
doi:
Substances chimiques
Plasminogen Activator Inhibitor 1
0
Fibrin
9001-31-4
Plasminogen
9001-91-6
Tissue Plasminogen Activator
EC 3.4.21.68
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : British Heart Foundation
ID : PG/08/127/2651
Pays : United Kingdom
Organisme : British Heart Foundation
ID : RG/16/10/32375
Pays : United Kingdom
Organisme : British Heart Foundation
ID : PG/15/82/31721
Pays : United Kingdom
Organisme : British Heart Foundation
ID : CH/09/002/26360
Pays : United Kingdom
Organisme : Wellcome Trust
ID : WT103782AIA
Pays : United Kingdom
Organisme : British Heart Foundation
ID : PG/04/131/18118
Pays : United Kingdom
Organisme : British Heart Foundation
ID : RE/18/5/34216
Pays : United Kingdom
Organisme : British Heart Foundation
ID : CH/09/002
Pays : United Kingdom
Organisme : British Heart Foundation
ID : FS/11/2/28579
Pays : United Kingdom
Organisme : British Heart Foundation
ID : PG/20/17/35050
Pays : United Kingdom
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