Proteomics of REPLICANT perfusate detects changes in the metastatic lymph node microenvironment.


Journal

NPJ breast cancer
ISSN: 2374-4677
Titre abrégé: NPJ Breast Cancer
Pays: United States
ID NLM: 101674891

Informations de publication

Date de publication:
05 Mar 2021
Historique:
received: 20 07 2020
accepted: 20 01 2021
entrez: 6 3 2021
pubmed: 7 3 2021
medline: 7 3 2021
Statut: epublish

Résumé

In breast cancer (BC), detecting low volumes of axillary lymph node (ALN) metastasis pre-operatively is difficult and novel biomarkers are needed. We recently showed that patient-derived ALNs can be sustained ex-vivo using normothermic perfusion. We now compare reactive (tumour-free; n = 5) and macrometastatic (containing tumour deposits >2 mm; n = 4) ALNs by combining whole section multiplex immunofluorescence with TMT-labelled LC-MS/MS of the circulating perfusate. Macrometastases contained significantly fewer B cells and T cells (CD4

Identifiants

pubmed: 33674617
doi: 10.1038/s41523-021-00227-7
pii: 10.1038/s41523-021-00227-7
pmc: PMC7935848
doi:

Types de publication

Journal Article

Langues

eng

Pagination

24

Subventions

Organisme : Cancer Research UK (CRUK)
ID : CRC598X
Organisme : Cancer Research UK (CRUK)
ID : Pioneer Award CRC598X
Organisme : Cancer Research UK (CRUK)
ID : Pioneer Award CRC598X

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Auteurs

Julia Stevenson (J)

The Breast Cancer Now Toby Robins Research Centre, The Institute of Cancer Research, London, UK.

Rachel Barrow-McGee (R)

The Breast Cancer Now Toby Robins Research Centre, The Institute of Cancer Research, London, UK.

Lu Yu (L)

Division of Cancer Biology, The Institute of Cancer Research, London, UK.

Angela Paul (A)

Division of Cancer Biology, The Institute of Cancer Research, London, UK.

David Mansfield (D)

Division of Radiotherapy and Imaging, The Institute of Cancer Research, London, UK.

Julie Owen (J)

King's Health Partners Cancer Biobank, Guy's Comprehensive Cancer Centre, London, UK.

Natalie Woodman (N)

King's Health Partners Cancer Biobank, Guy's Comprehensive Cancer Centre, London, UK.

Rachael Natrajan (R)

The Breast Cancer Now Toby Robins Research Centre, The Institute of Cancer Research, London, UK.

Syed Haider (S)

The Breast Cancer Now Toby Robins Research Centre, The Institute of Cancer Research, London, UK.

Cheryl Gillett (C)

King's Health Partners Cancer Biobank, Guy's Comprehensive Cancer Centre, London, UK.

Andrew Tutt (A)

The Breast Cancer Now Toby Robins Research Centre, The Institute of Cancer Research, London, UK.

Sarah E Pinder (SE)

School of Cancer and Pharmaceutical Sciences, King's College London, Guy's Comprehensive Cancer Centre, London, UK.

Jyoti Choudary (J)

Division of Cancer Biology, The Institute of Cancer Research, London, UK.

Kalnisha Naidoo (K)

The Breast Cancer Now Toby Robins Research Centre, The Institute of Cancer Research, London, UK. kalnisha.naidoo1@nhs.net.
Department of Cellular Pathology, Imperial College Healthcare NHS Trust, Charing Cross Hospital, London, UK. kalnisha.naidoo1@nhs.net.

Classifications MeSH