Surface activation with oxygen plasma promotes osteogenesis with enhanced extracellular matrix formation in three-dimensional microporous scaffolds.
Alkaline Phosphatase
/ metabolism
Animals
Calcification, Physiologic
/ drug effects
Cell Adhesion
/ drug effects
Cell Differentiation
/ drug effects
Cell Proliferation
/ drug effects
Collagen
/ metabolism
Dioxanes
/ pharmacology
Extracellular Matrix
/ drug effects
Hydrophobic and Hydrophilic Interactions
Mesenchymal Stem Cells
/ cytology
Nanoparticles
/ chemistry
Osteogenesis
/ drug effects
Oxygen
/ pharmacology
Plasma Gases
/ pharmacology
Porosity
RNA, Messenger
/ genetics
Rats, Inbred Lew
Surface Properties
Tissue Scaffolds
/ chemistry
biomaterials
bone tissue engineering
lactide-TMC
mesenchymal stem cells
osteogenic differentiation
plasma activation
Journal
Journal of biomedical materials research. Part A
ISSN: 1552-4965
Titre abrégé: J Biomed Mater Res A
Pays: United States
ID NLM: 101234237
Informations de publication
Date de publication:
09 2021
09 2021
Historique:
revised:
03
02
2021
received:
23
06
2020
accepted:
10
02
2021
pubmed:
7
3
2021
medline:
24
2
2022
entrez:
6
3
2021
Statut:
ppublish
Résumé
Various types of synthetic polyesters have been developed as biomaterials for tissue engineering. These materials commonly possess biodegradability, biocompatibility, and formability, which are preferable properties for bone regeneration. The major challenge of using synthetic polyesters is the result of low cell affinity due to their hydrophobic nature, which hinders efficient cell seeding and active cell dynamics. To improve wettability, plasma treatment is widely used in industry. Here, we performed surface activation with oxygen plasma to hydrophobic copolymers, poly(l-lactide-co-trimethylene carbonate), which were shaped in 2D films and 3D microporous scaffolds, and then we evaluated the resulting surface properties and the cellular responses of rat bone marrow stem cells (rBMSC) to the material. Using scanning electron microscopy and Fourier-transform infrared spectroscopy, we demonstrated that short-term plasma treatment increased nanotopographical surface roughness and wettability with minimal change in surface chemistry. On treated surfaces, initial cell adhesion and elongation were significantly promoted, and seeding efficiency was improved. In an osteoinductive environment, rBMSC on plasma-treated scaffolds exhibited accelerated osteogenic differentiation with osteogenic markers including RUNX2, osterix, bone sialoprotein, and osteocalcin upregulated, and a greater amount of collagen matrix and mineral deposition were found. This study shows the utility of plasma surface activation for polymeric scaffolds in bone tissue engineering.
Substances chimiques
Dioxanes
0
Plasma Gases
0
RNA, Messenger
0
trimethylene carbonate
4316AQ174Q
Collagen
9007-34-5
dilactide
95-96-5
Alkaline Phosphatase
EC 3.1.3.1
Oxygen
S88TT14065
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1560-1574Informations de copyright
© 2021 The Authors. Journal of Biomedical Materials Research Part A published by Wiley Periodicals LLC.
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