miR-34a-5p might have an important role for inducing apoptosis by down-regulation of SNAI1 in apigenin-treated lung cancer cells.
A549 Cells
Apigenin
/ pharmacology
Apoptosis
/ drug effects
Cell Survival
/ drug effects
Down-Regulation
/ drug effects
Gene Expression Regulation, Neoplastic
/ drug effects
Humans
Lung Neoplasms
/ genetics
MicroRNAs
/ genetics
Models, Biological
RNA, Messenger
/ genetics
Snail Family Transcription Factors
/ genetics
Up-Regulation
/ drug effects
Apigenin
Apoptosis
Human lung cancer cell A549
SNAI1 (SNAIL)
miR-34a-5p
Journal
Molecular biology reports
ISSN: 1573-4978
Titre abrégé: Mol Biol Rep
Pays: Netherlands
ID NLM: 0403234
Informations de publication
Date de publication:
Mar 2021
Mar 2021
Historique:
received:
09
01
2021
accepted:
25
02
2021
pubmed:
7
3
2021
medline:
20
5
2021
entrez:
6
3
2021
Statut:
ppublish
Résumé
Apigenin is a flavonoid with antioxidant and anticancer effects. It has been reported that apigenin inhibits proliferation, migration, and invasion and induces apoptosis in cultured lung cancer cells. However, there is little information on the involvement of microRNAs (miRNAs) in its effects. miRNA microarray analysis and polymerase-chain-reaction analysis of miRNAs revealed that treatment of human lung cancer A549 cells with apigenin up-regulated the level of miR-34a-5p. Furthermore, mRNA microarray analysis and the results of three microRNA target prediction tools showed that Snail Family Transcriptional Repressor 1 (SNAI1), which inhibits the induction of apoptosis, had its mRNA expression down-regulated in A549 cells treated with apigenin. Our findings suggest that apigenin might induce apoptosis by down-regulation of SNAI1 through up-regulation of miR-34a-5p in A549 cells.
Identifiants
pubmed: 33675467
doi: 10.1007/s11033-021-06255-7
pii: 10.1007/s11033-021-06255-7
pmc: PMC8060201
doi:
Substances chimiques
MIRN34 microRNA, human
0
MicroRNAs
0
RNA, Messenger
0
SNAI1 protein, human
0
Snail Family Transcription Factors
0
Apigenin
7V515PI7F6
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2291-2297Références
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