Aptamer-based approaches to detect nucleolin in prostate cancer.

Aptamers, Nucleotide Humans Leukocytes, Mononuclear / metabolism Male Oligodeoxyribonucleotides Phosphoproteins / analysis Prostatic Neoplasms / diagnosis RNA-Binding Proteins / analysis Nucleolin
AS1411-N5 G-quadruplex Microfluidic Nucleolin Prostate cancer

Journal

Talanta
ISSN: 1873-3573
Titre abrégé: Talanta
Pays: Netherlands
ID NLM: 2984816R

Informations de publication

Date de publication:
01 May 2021
Historique:
received: 17 08 2020
revised: 15 12 2020
accepted: 17 12 2020
entrez: 7 3 2021
pubmed: 8 3 2021
medline: 15 5 2021
Statut: ppublish

Résumé

We have investigated the expression of nucleolin (NCL) in liquid biopsies of prostate cancer (PCa) patients and healthy controls to determine its correlation with tumor prognosis. To detect NCL we used a modified AS1411 aptamer designated by AS1411-N5. In presence of NCL, AS1411-N5 increases the fluorescence by assuming a G-quadruplex (G4) structure, while in the absence of NCL the fluorescence signal remains quenched. The structural characterization of AS1411-N5 was performed by biophysical studies, which demonstrated the formation of G4 parallel conformation in the presence of 100 mM K

Identifiants

DOI: 10.1016/j.talanta.2020.122037 PMID: 33676639
pubmed: 33676639
pii: S0039-9140(20)31328-X
doi: 10.1016/j.talanta.2020.122037
pii:
doi:

Substances chimiques

Aptamers, Nucleotide 0
Oligodeoxyribonucleotides 0
Phosphoproteins 0
RNA-Binding Proteins 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

122037

Informations de copyright

Copyright © 2020 Elsevier B.V. All rights reserved.

Auteurs

André Miranda (A)

CICS-UBI - Centro de Investigação em Ciências da Saúde, Universidade da Beira Interior, Av. Infante D. Henrique, Covilhã, Portugal.

Tiago Santos (T)

CICS-UBI - Centro de Investigação em Ciências da Saúde, Universidade da Beira Interior, Av. Infante D. Henrique, Covilhã, Portugal.

Josué Carvalho (J)

CICS-UBI - Centro de Investigação em Ciências da Saúde, Universidade da Beira Interior, Av. Infante D. Henrique, Covilhã, Portugal.

Daniela Alexandre (D)

CICS-UBI - Centro de Investigação em Ciências da Saúde, Universidade da Beira Interior, Av. Infante D. Henrique, Covilhã, Portugal.

Andreia Jardim (A)

CICS-UBI - Centro de Investigação em Ciências da Saúde, Universidade da Beira Interior, Av. Infante D. Henrique, Covilhã, Portugal; Instituto de Engenharia de Sistemas e Computadores - Microsistemas e Nanotecnologias (INESC MN) and IN - Institute of Nanoscience and Nanotechnology, Lisbon, Portugal.

CatarinaR F Caneira (CF)

Instituto de Engenharia de Sistemas e Computadores - Microsistemas e Nanotecnologias (INESC MN) and IN - Institute of Nanoscience and Nanotechnology, Lisbon, Portugal.

Vírgilio Vaz (V)

Serviço de Urologia do Centro Hospitalar Universitário Cova da Beira (CHUCB), Covilhã, Portugal.

Bruno Pereira (B)

Faculdade de Ciências da Saúde, Universidade da Beira Interior (FCS-UBI), Covilhã, Portugal; Instituto Português de Oncologia (IPO), Coimbra, Portugal.

Ricardo Godinho (R)

Instituto Português de Oncologia (IPO), Coimbra, Portugal.

Duarte Brito (D)

Instituto Português de Oncologia (IPO), Coimbra, Portugal.

Virgínia Chu (V)

Instituto de Engenharia de Sistemas e Computadores - Microsistemas e Nanotecnologias (INESC MN) and IN - Institute of Nanoscience and Nanotechnology, Lisbon, Portugal.

João P Conde (JP)

Instituto de Engenharia de Sistemas e Computadores - Microsistemas e Nanotecnologias (INESC MN) and IN - Institute of Nanoscience and Nanotechnology, Lisbon, Portugal; Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Lisbon, Portugal.

Carla Cruz (C)

CICS-UBI - Centro de Investigação em Ciências da Saúde, Universidade da Beira Interior, Av. Infante D. Henrique, Covilhã, Portugal. Electronic address: carlacruz@fcsaude.ubi.pt.

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Classifications MeSH

questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Nucléotides › Polynucléotides › Oligonucléotides › Aptamères nucléotidiques › Aptamer-based approaches to detect nucleolin...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Aptamer-based approaches to detect nucleolin...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Leucocytes › Agranulocytes › Aptamer-based approaches to detect nucleolin...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Nucléotides › Polynucléotides › Oligonucléotides › Oligodésoxyribonucléotides › Aptamer-based approaches to detect nucleolin...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Phosphoprotéines › Aptamer-based approaches to detect nucleolin...
questionsmedicales.fr › Maladies urogénitales › Tumeurs de l'appareil urogénital › Tumeurs de l'appareil génital mâle › Tumeurs de la prostate › Aptamer-based approaches to detect nucleolin...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Nucléoprotéines › Protéines de liaison à l'ARN › Aptamer-based approaches to detect nucleolin...