Comparative study of autofluorescence in flat and tapered optical fibers towards application in depth-resolved fluorescence lifetime photometry in brain tissue.
Journal
Biomedical optics express
ISSN: 2156-7085
Titre abrégé: Biomed Opt Express
Pays: United States
ID NLM: 101540630
Informations de publication
Date de publication:
01 Feb 2021
01 Feb 2021
Historique:
received:
16
09
2020
revised:
21
12
2020
accepted:
29
12
2020
entrez:
8
3
2021
pubmed:
9
3
2021
medline:
9
3
2021
Statut:
epublish
Résumé
As the scientific community seeks efficient optical neural interfaces with sub-cortical structures of the mouse brain, a wide set of technologies and methods is being developed to monitor cellular events through fluorescence signals generated by genetically encoded molecules. Among these technologies, tapered optical fibers (TFs) take advantage of the modal properties of narrowing waveguides to enable both depth-resolved and wide-volume light collection from scattering tissue, with minimized invasiveness with respect to standard flat fiber stubs (FFs). However, light guided in patch cords as well as in FFs and TFs can result in autofluorescence (AF) signal, which can act as a source of time-variable noise and limit their application to probe fluorescence lifetime
Identifiants
pubmed: 33680555
doi: 10.1364/BOE.410244
pii: 410244
pmc: PMC7901336
doi:
Types de publication
Journal Article
Langues
eng
Pagination
993-1010Subventions
Organisme : European Research Council
ID : 677683
Pays : International
Organisme : European Research Council
ID : 692943
Pays : International
Organisme : NINDS NIH HHS
ID : U01 NS094190
Pays : United States
Organisme : NINDS NIH HHS
ID : UF1 NS108177
Pays : United States
Informations de copyright
© 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.
Déclaration de conflit d'intérêts
L.S., M.D.V., B.L.S. and F. Pisanello are founders and hold private equity in Optogenix, a company that develops, produces and sells technologies to deliver light into the brain. Tapered fibers commercially available from Optogenix were used as tools in the research.
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