A System-Agnostic, Adaptable and Extensible Animal Support Cradle System for Cardio-Respiratory-Synchronised, and Other, Multi-Modal Imaging of Small Animals.

handling apparatus imaging-compatible multi-modal imaging multi-vendor optimisation

Journal

Tomography (Ann Arbor, Mich.)
ISSN: 2379-139X
Titre abrégé: Tomography
Pays: Switzerland
ID NLM: 101671170

Informations de publication

Date de publication:
03 2021
Historique:
received: 29 01 2021
accepted: 01 02 2021
entrez: 8 3 2021
pubmed: 9 3 2021
medline: 3 8 2021
Statut: epublish

Résumé

Standardisation of animal handling procedures for a wide range of preclinical imaging scanners will improve imaging performance and reproducibility of scientific data. Whilst there has been significant effort in defining how well scanners should operate and how in vivo experimentation should be practised, there is little detail on how to achieve optimal scanner performance with best practices in animal welfare. Here, we describe a system-agnostic, adaptable and extensible animal support cradle system for cardio-respiratory-synchronised, and other, multi-modal imaging of small animals. The animal support cradle can be adapted on a per application basis and features integrated tubing for anaesthetic and tracer delivery, an electrically driven rectal temperature maintenance system and respiratory and cardiac monitoring. Through a combination of careful material and device selection, we have described an approach that allows animals to be transferred whilst under general anaesthesia between any of the tomographic scanners we currently or have previously operated. The set-up is minimally invasive, cheap and easy to implement and for multi-modal, multi-vendor imaging of small animals.

Identifiants

pubmed: 33681462
doi: 10.3390/tomography7010004
pii: tomography7010004
pmc: PMC7934707
doi:

Substances chimiques

Anesthetics 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

39-54

Informations de copyright

© 2021 by the authors.

Déclaration de conflit d'intérêts

Conflicts of InterestThe authors declare no conflict of interest.

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Auteurs

Veerle Kersemans (V)

Preclinical Imaging Scientific Research Facility, Department of Oncology, University of Oxford, Oxford OX3 7DQ, UK.

Stuart Gilchrist (S)

Preclinical Imaging Scientific Research Facility, Department of Oncology, University of Oxford, Oxford OX3 7DQ, UK.

Philip Danny Allen (PD)

Preclinical Imaging Scientific Research Facility, Department of Oncology, University of Oxford, Oxford OX3 7DQ, UK.

Sheena Wallington (S)

Preclinical Imaging Scientific Research Facility, Department of Oncology, University of Oxford, Oxford OX3 7DQ, UK.

Paul Kinchesh (P)

Preclinical Imaging Scientific Research Facility, Department of Oncology, University of Oxford, Oxford OX3 7DQ, UK.

John Prentice (J)

Preclinical Imaging Scientific Research Facility, Department of Oncology, University of Oxford, Oxford OX3 7DQ, UK.

Martin Tweedie (M)

Department of Materials, Quantum Information Processing Interdisciplinary Research Collaboration, University of Oxford, Parks Road, Oxford OX1 3PH, UK.

Jamie H Warner (JH)

Department of Materials, Quantum Information Processing Interdisciplinary Research Collaboration, University of Oxford, Parks Road, Oxford OX1 3PH, UK.

Sean C Smart (SC)

Preclinical Imaging Scientific Research Facility, Department of Oncology, University of Oxford, Oxford OX3 7DQ, UK.

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Classifications MeSH