Ground state depletion microscopy as a tool for studying microglia-synapse interactions.

Aged Aged, 80 and over Alzheimer Disease / pathology Animals Antibodies Female Humans Larva Male Mice Microglia / physiology Microscopy / methods Microscopy, Confocal Middle Aged Presynaptic Terminals / physiology Synapses / physiology Tissue Fixation Zebrafish
Alzheimer disease RRID:AB_141874 RRID:AB_2199013 RRID:AB_2286948 RRID:AB_2534072 RRID:AB_2534076 RRID:AB_2535731 RRID:AB_2536183 RRID:AB_2633277 RRID:AB_324660 RRID:AB_839504 RRID:SCR_001622 RRID:SCR_002285 RRID:SCR_002798 RRID:SCR_013673 RRID:SCR_013726 ground state depletion followed by individual molecule return microscopy microglia post-mortem archival human brain tissue super-resolution microscopy synapses zebrafish

Journal

Journal of neuroscience research
ISSN: 1097-4547
Titre abrégé: J Neurosci Res
Pays: United States
ID NLM: 7600111

Informations de publication

Date de publication:
06 2021
Historique:
revised: 02 02 2021
received: 22 07 2020
accepted: 06 02 2021
pubmed: 9 3 2021
medline: 29 1 2022
entrez: 8 3 2021
Statut: ppublish

Résumé

Ground state depletion followed by individual molecule return microscopy (GSDIM) has been used in the past to study the nanoscale distribution of protein co-localization in living cells. We now demonstrate the successful application of GSDIM to archival human brain tissue sections including from Alzheimer's disease cases as well as experimental tissue samples from mouse and zebrafish larvae. Presynaptic terminals and microglia and their cell processes were visualized at a resolution beyond diffraction-limited light microscopy, allowing clearer insights into their interactions in situ. The procedure described here offers time and cost savings compared to electron microscopy and opens the spectrum of molecular imaging using antibodies and super-resolution microscopy to the analysis of routine formalin-fixed paraffin sections of archival human brain. The investigation of microglia-synapse interactions in dementia will be of special interest in this context.

Identifiants

DOI: 10.1002/jnr.24819 PMID: 33682204 PMC: PMC8251743
pubmed: 33682204
doi: 10.1002/jnr.24819
pmc: PMC8251743
doi:

Substances chimiques

Antibodies 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

1515-1532

Subventions

Organisme : NIAAA NIH HHS
ID : R24 AA012725
Pays : United States
Organisme : NIAAA NIH HHS
ID : R28 AA012725
Pays : United States
Organisme : NIAAA NIH HHS
ID : R01 AA012725
Pays : United States

Informations de copyright

© 2021 The Authors. Journal of Neuroscience Research published by Wiley Periodicals LLC.

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Auteurs

Patrick Jarmo Paasila (PJ)

Faculty of Medicine and Health, Charles Perkins Centre and School of Medical Sciences, The University of Sydney, Camperdown, NSW, Australia.

Sandra Y Y Fok (SYY)

Biomedical Imaging Facility, Mark Wainwright Analytical Centre, University of New South Wales Sydney, Kensington, NSW, Australia.

Neftali Flores-Rodriguez (N)

Charles Perkins Centre, Sydney Microscopy and Microanalysis, The University of Sydney, Camperdown, NSW, Australia.

Sujata Sajjan (S)

Faculty of Medicine and Health, Brain and Mind Centre, The University of Sydney, Camperdown, NSW, Australia.

Adam J Svahn (AJ)

Faculty of Medicine and Health, Brain and Mind Centre, The University of Sydney, Camperdown, NSW, Australia.

Claude V Dennis (CV)

Faculty of Medicine and Health, Charles Perkins Centre and School of Medical Sciences, The University of Sydney, Camperdown, NSW, Australia.

R M Damian Holsinger (RMD)

Faculty of Medicine and Health, Brain and Mind Centre, The University of Sydney, Camperdown, NSW, Australia.

Jillian J Kril (JJ)

Faculty of Medicine and Health, Charles Perkins Centre and School of Medical Sciences, The University of Sydney, Camperdown, NSW, Australia.

Thomas S Becker (TS)

Faculty of Medicine and Health, Brain and Mind Centre, The University of Sydney, Camperdown, NSW, Australia.

Richard B Banati (RB)

Faculty of Medicine and Health, Brain and Mind Centre, The University of Sydney, Camperdown, NSW, Australia.
Life Sciences, Australian Nuclear Science and Technology Organisation, Kirrawee, NSW, Australia.

Greg T Sutherland (GT)

Faculty of Medicine and Health, Charles Perkins Centre and School of Medical Sciences, The University of Sydney, Camperdown, NSW, Australia.

Manuel B Graeber (MB)

Faculty of Medicine and Health, Brain and Mind Centre, The University of Sydney, Camperdown, NSW, Australia.

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