Targeted knockdown of ribulose-1, 5-bisphosphate carboxylase-oxygenase in rice mesophyll cells.

Gene Knockdown Techniques Mesophyll Cells / metabolism Oryza / genetics Photosynthesis Plant Leaves / growth & development Ribulose-Bisphosphate Carboxylase / genetics
Antisense C(4) rice Oryza sativa RBCS Rubisco

Journal

Journal of plant physiology
ISSN: 1618-1328
Titre abrégé: J Plant Physiol
Pays: Germany
ID NLM: 9882059

Informations de publication

Date de publication:
May 2021
Historique:
received: 28 10 2020
revised: 06 02 2021
accepted: 21 02 2021
pubmed: 9 3 2021
medline: 29 5 2021
entrez: 8 3 2021
Statut: ppublish

Résumé

We generated antisense constructs targeting two of the five Rubisco small subunit genes (OsRBCS2 and 4) which account for between 30-40 % of the RBCS transcript abundance in leaf blades. The constructs were driven by a maize phosphoenolpyruvate carboxylase (PEPC) promoter known to have enriched expression in mesophyll cells (MCs). In the resulting lines leaf, Rubisco protein content was reduced by between 30-50 % and CO

Identifiants

DOI: 10.1016/j.jplph.2021.153395 PMID: 33684805 PMC: PMC8090977
pubmed: 33684805
pii: S0176-1617(21)00034-1
doi: 10.1016/j.jplph.2021.153395
pmc: PMC8090977
pii:
doi:

Substances chimiques

Ribulose-Bisphosphate Carboxylase EC 4.1.1.39

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

153395

Informations de copyright

Copyright © 2021 The Authors. Published by Elsevier GmbH.. All rights reserved.

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Auteurs

Chirag Maheshwari (C)

C4Rice Centre, International Rice Research Institute (IRRI), Los Baños, Philippines.

Robert A Coe (RA)

C4Rice Centre, International Rice Research Institute (IRRI), Los Baños, Philippines.

Shanta Karki (S)

C4Rice Centre, International Rice Research Institute (IRRI), Los Baños, Philippines.

Sarah Covshoff (S)

Department of Plant Sciences, University of Cambridge, Cambridge, CB2 3EA, United Kingdom.

Ronald Tapia (R)

C4Rice Centre, International Rice Research Institute (IRRI), Los Baños, Philippines.

Aruna Tyagi (A)

Division of Biochemistry, ICAR-Indian Agricultural Research Institute, New Delhi, India.

Julian M Hibberd (JM)

Department of Plant Sciences, University of Cambridge, Cambridge, CB2 3EA, United Kingdom.

Robert T Furbank (RT)

ARC Centre of Excellence for Translational Photosynthesis, Research School of Biology, The Australian National University, Acton, 2601, Australia.

William Paul Quick (WP)

C4Rice Centre, International Rice Research Institute (IRRI), Los Baños, Philippines; Department of Animal and Plant Sciences, University of Sheffield, Sheffield, S10 2TN, United Kingdom.

Hsiang-Chun Lin (HC)

C4Rice Centre, International Rice Research Institute (IRRI), Los Baños, Philippines. Electronic address: h.lin@irri.org.

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Ciblage de gène › Techniques de knock-down de gènes › Targeted knockdown of ribulose-1,...
questionsmedicales.fr › Structures de plante › Parties aériennes de plante › Feuilles de plante › Cellules du mésophylle › Targeted knockdown of ribulose-1,...
questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Poaceae › Oryza › Targeted knockdown of ribulose-1,...
questionsmedicales.fr › Phénomènes physiologiques des plantes › Photosynthèse › Targeted knockdown of ribulose-1,...
questionsmedicales.fr › Structures de plante › Parties aériennes de plante › Feuilles de plante › Targeted knockdown of ribulose-1,...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines végétales › Protéines chloroplastiques › Ribulose bisphosphate carboxylase › Targeted knockdown of ribulose-1,...