A flat embedding method for transmission electron microscopy reveals an unknown mechanism of tetracycline.
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
08 03 2021
08 03 2021
Historique:
received:
05
12
2019
accepted:
05
02
2021
entrez:
9
3
2021
pubmed:
10
3
2021
medline:
10
8
2021
Statut:
epublish
Résumé
Transmission electron microscopy of cell sample sections is a popular technique in microbiology. Currently, ultrathin sectioning is done on resin-embedded cell pellets, which consumes milli- to deciliters of culture and results in sections of randomly orientated cells. This is problematic for rod-shaped bacteria and often precludes large-scale quantification of morphological phenotypes due to the lack of sufficient numbers of longitudinally cut cells. Here we report a flat embedding method that enables observation of thousands of longitudinally cut cells per single section and only requires microliter culture volumes. We successfully applied this technique to Bacillus subtilis, Escherichia coli, Mycobacterium bovis, and Acholeplasma laidlawii. To assess the potential of the technique to quantify morphological phenotypes, we monitored antibiotic-induced changes in B. subtilis cells. Surprisingly, we found that the ribosome inhibitor tetracycline causes membrane deformations. Further investigations showed that tetracycline disturbs membrane organization and localization of the peripheral membrane proteins MinD, MinC, and MreB. These observations are not the result of ribosome inhibition but constitute a secondary antibacterial activity of tetracycline that so far has defied discovery.
Identifiants
pubmed: 33686188
doi: 10.1038/s42003-021-01809-8
pii: 10.1038/s42003-021-01809-8
pmc: PMC7940657
doi:
Substances chimiques
Anti-Bacterial Agents
0
Bacterial Proteins
0
Membrane Proteins
0
MinC protein, Bacteria
0
Tetracycline
F8VB5M810T
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Video-Audio Media
Langues
eng
Sous-ensembles de citation
IM
Pagination
306Références
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