Engineered SARS-CoV-2 receptor binding domain improves immunogenicity in mice and elicits protective immunity in hamsters.

Journal

bioRxiv : the preprint server for biology
Titre abrégé: bioRxiv
Pays: United States
ID NLM: 101680187

Informations de publication

Date de publication:
04 Mar 2021
Historique:
pubmed: 11 3 2021
medline: 11 3 2021
entrez: 10 3 2021
Statut: epublish

Résumé

Global containment of COVID-19 still requires accessible and affordable vaccines for low- and middle-income countries (LMICs).

Identifiants

DOI: 10.1101/2021.03.03.433558 PMID: 33688647 PMC: PMC7941618
pubmed: 33688647
doi: 10.1101/2021.03.03.433558
pmc: PMC7941618
pii:
doi:

Types de publication

Preprint

Langues

eng

Subventions

Organisme : Bill & Melinda Gates Foundation
ID : INV-002740
Pays : United States
Organisme : Bill & Melinda Gates Foundation
ID : INV-006131
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA014051
Pays : United States
Organisme : NIAID NIH HHS
ID : T32 AI007387
Pays : United States

Commentaires et corrections

Type : UpdateIn

Déclaration de conflit d'intérêts

Competing interests L.E.C., K.R.L., and J.C.L. have filed patents related to the InSCyT system and methods. N.C.D., S.R.A., and J.C.L. have filed a patent related to the RBD-L452K-F490W sequence. K.R.L., L.E.C., and M.K.T. are current employees at Sunflower Therapeutics PBC. J.C.L. has interests in Sunflower Therapeutics PBC, Pfizer, Honeycomb Biotechnologies, OneCyte Biotechnologies, QuantumCyte, Amgen, and Repligen. J.C.L’s interests are reviewed and managed under MIT’s policies for potential conflicts of interest. J.M.S. is an employee of the Bill & Melinda Gates Medical Research Institute. H.K. is an employee of the Bill & Melinda Gates Foundation.

Auteurs

Neil C Dalvie (NC)

Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.

Sergio A Rodriguez-Aponte (SA)

The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.

Brittany L Hartwell (BL)

The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Ragon Institute of MGH, MIT, Harvard, Cambridge, MA 02139, USA.

Lisa H Tostanoski (LH)

Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.

Andrew M Biedermann (AM)

Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.

Laura E Crowell (LE)

Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.

Kawaljit Kaur (K)

Department of Pharmaceutical Chemistry, Vaccine Analytics and Formulation Center, University of Kansas, Lawrence, Kansas, 66047, United States.

Ozan Kumru (O)

Department of Pharmaceutical Chemistry, Vaccine Analytics and Formulation Center, University of Kansas, Lawrence, Kansas, 66047, United States.

Lauren Carter (L)

Department of Biochemistry, University of Washington, Seattle, WA 98195, USA.
Institute for Protein Design, University of Washington, Seattle, WA 98195, USA.

Jingyou Yu (J)

Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.

Aiquan Chang (A)

Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
Harvard Medical School, Boston, MA 02115, USA.

Katherine McMahan (K)

Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.

Thomas Courant (T)

Vaccine Formulation Institute, 1228 Plan-Les-Ouates, Geneva, Switzerland.

Celia Lebas (C)

Vaccine Formulation Institute, 1228 Plan-Les-Ouates, Geneva, Switzerland.

Ashley A Lemnios (AA)

The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.

Kristen A Rodrigues (KA)

The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Ragon Institute of MGH, MIT, Harvard, Cambridge, MA 02139, USA.
Harvard-MIT Health Sciences and Technology, Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

Murillo Silva (M)

The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.

Ryan S Johnston (RS)

The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.

Christopher A Naranjo (CA)

The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.

Mary Kate Tracey (MK)

The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.

Joseph R Brady (JR)

Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.

Charles A Whittaker (CA)

The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.

Dongsoo Yun (D)

The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.

Swagata Kar (S)

Bioqual, Inc., Rockville, MD 20850, USA.

Maciel Porto (M)

Bioqual, Inc., Rockville, MD 20850, USA.

Megan Lok (M)

Bioqual, Inc., Rockville, MD 20850, USA.

Hanne Andersen (H)

Bioqual, Inc., Rockville, MD 20850, USA.

Mark G Lewis (MG)

Bioqual, Inc., Rockville, MD 20850, USA.

Kerry R Love (KR)

Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.

Danielle L Camp (DL)

The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.

Judith Maxwell Silverman (JM)

Bill & Melinda Gates Medical Research Institute, Cambridge, MA 02139, USA.

Harry Kleanthous (H)

Bill&Melinda Gates Foundation, Seattle, WA 98109, USA.

Sangeeta B Joshi (SB)

Department of Pharmaceutical Chemistry, Vaccine Analytics and Formulation Center, University of Kansas, Lawrence, Kansas, 66047, United States.

David B Volkin (DB)

Department of Pharmaceutical Chemistry, Vaccine Analytics and Formulation Center, University of Kansas, Lawrence, Kansas, 66047, United States.

Patrice M Dubois (PM)

Vaccine Formulation Institute, 1228 Plan-Les-Ouates, Geneva, Switzerland.

Nicolas Collin (N)

Vaccine Formulation Institute, 1228 Plan-Les-Ouates, Geneva, Switzerland.

Neil P King (NP)

Department of Biochemistry, University of Washington, Seattle, WA 98195, USA.
Institute for Protein Design, University of Washington, Seattle, WA 98195, USA.

Dan H Barouch (DH)

Ragon Institute of MGH, MIT, Harvard, Cambridge, MA 02139, USA.
Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
Harvard Medical School, Boston, MA 02115, USA.
Massachusetts Consortium on Pathogen Readiness, Boston, MA 02115, USA.

Darrell J Irvine (DJ)

The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Ragon Institute of MGH, MIT, Harvard, Cambridge, MA 02139, USA.
Howard Hughes Medical Institute, Chevy Chase, MD 20815, USA.

J Christopher Love (JC)

Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.

Classifications MeSH