Development and Characterization of Nanobodies Targeting the Kupffer Cell.
Clec4F
Kupffer cells
epitope binning
nanobody
x-ray crystallography
Journal
Frontiers in immunology
ISSN: 1664-3224
Titre abrégé: Front Immunol
Pays: Switzerland
ID NLM: 101560960
Informations de publication
Date de publication:
2021
2021
Historique:
received:
16
12
2020
accepted:
01
02
2021
entrez:
11
3
2021
pubmed:
12
3
2021
medline:
23
9
2021
Statut:
epublish
Résumé
Nanobodies that are derived from single-chain antibodies of camelids have served as powerful tools in diagnostics, therapeutics and investigation of membrane receptors' structure and function. In this study, we developed a series of nanobodies by a phage display screening building from lymphocytes isolated from an alpaca immunized with recombinant mouse Kupffer cell receptor Clec4F, which is involved in pathogen recognition by binding to galactose and N-acetylgalactosamine. Bio-panning selections retrieved 14 different nanobodies against Clec4F with an affinity ranging from 0.2 to 2 nM as determined by SPR. Those nanobodies mainly recognize 4 different epitopes as analyzed
Identifiants
pubmed: 33692811
doi: 10.3389/fimmu.2021.641819
pmc: PMC7937711
doi:
Substances chimiques
DCIR4 protein, mouse
0
Lectins, C-Type
0
Single-Domain Antibodies
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
641819Informations de copyright
Copyright © 2021 Zheng, Zhou, Ouyang, Zhang, Wang, Muyldermans, Van Ginderachter, Devoogdt, Wen, Schoonooghe and Raes.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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