Integrated Approach for the Diagnosis of Shiga Toxin-Producing Escherichia coli Infections in Humans.
Anti-lipopolysaccharide antibodies
ELISA
Real time PCR
STEC isolation
Serology
Shiga toxin
Vero cell assay
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
11
3
2021
pubmed:
12
3
2021
medline:
2
4
2021
Statut:
ppublish
Résumé
Shiga toxin-producing Escherichia coli (STEC) are human pathogens causing severe diseases, such as hemorrhagic colitis and the hemolytic uremic syndrome. The prompt diagnosis of STEC infection is of primary importance to drive the most appropriate patient's management procedures. The methods to diagnose STEC infections include both direct isolation of the STEC from stool samples and the identification of indirect evidences based on molecular, phenotypic, and serological applications. Here, the procedures in use at the Italian Reference Laboratory for E. coli infections are described.
Identifiants
pubmed: 33704747
doi: 10.1007/978-1-0716-1339-9_1
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1-17Références
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