Bacterial Growth Inhibition Screen (BGIS): harnessing recombinant protein toxicity for rapid and unbiased interrogation of protein function.
Animals
Bias
Biocatalysis
Chromosomal Proteins, Non-Histone
/ chemistry
Drosophila Proteins
/ chemistry
Escherichia coli
/ drug effects
Humans
Loss of Function Mutation
Oncogene Proteins
/ chemistry
Peptide Fragments
/ chemistry
Poly-ADP-Ribose Binding Proteins
/ chemistry
Protein Domains
/ genetics
RNA
/ metabolism
RNA-Binding Proteins
/ chemistry
Receptors, Eph Family
/ chemistry
Recombinant Fusion Proteins
/ chemistry
Reproducibility of Results
Time Factors
Toxicity Tests
/ methods
Escherichia coli
DEK
RNA-binding domain
oncogene
protein domain
protein function
recombinant protein toxicity
Journal
FEBS letters
ISSN: 1873-3468
Titre abrégé: FEBS Lett
Pays: England
ID NLM: 0155157
Informations de publication
Date de publication:
05 2021
05 2021
Historique:
revised:
28
02
2021
received:
02
02
2021
accepted:
02
03
2021
pubmed:
12
3
2021
medline:
16
7
2021
entrez:
11
3
2021
Statut:
ppublish
Résumé
In two proof-of-concept studies, we established and validated the Bacterial Growth Inhibition Screen (BGIS), which explores recombinant protein toxicity in Escherichia coli as a largely overlooked and alternative means for basic characterization of functional eukaryotic protein domains. By applying BGIS, we identified an unrecognized RNA-interacting domain in the DEK oncoprotein (this study) and successfully combined BGIS with random mutagenesis as a screening tool for loss-of-function mutants of the DNA modulating domain of DEK [1]. Collectively, our findings shed new light on the phenomenon of recombinant protein toxicity in E. coli. Given the easy and rapid implementation and wide applicability, BGIS will extend the repertoire of basic methods for the identification, analysis and unbiased manipulation of proteins.
Identifiants
pubmed: 33704777
doi: 10.1002/1873-3468.14072
doi:
Substances chimiques
Chromosomal Proteins, Non-Histone
0
DEK protein, human
0
Drosophila Proteins
0
Oncogene Proteins
0
Peptide Fragments
0
Poly-ADP-Ribose Binding Proteins
0
RNA-Binding Proteins
0
Recombinant Fusion Proteins
0
RNA
63231-63-0
Dek protein, Drosophila
EC 2.7.10.1
Receptors, Eph Family
EC 2.7.10.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Validation Study
Langues
eng
Sous-ensembles de citation
IM
Pagination
1422-1437Informations de copyright
© 2021 Federation of European Biochemical Societies.
Références
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