Identification of ribosomal protein L30 as an uncharacterized antimicrobial protein.
Amphioxus
Antimicrobial peptides
Moonlighting protein
Ribosomal protein L30
Journal
Developmental and comparative immunology
ISSN: 1879-0089
Titre abrégé: Dev Comp Immunol
Pays: United States
ID NLM: 7708205
Informations de publication
Date de publication:
07 2021
07 2021
Historique:
received:
19
01
2021
revised:
02
03
2021
accepted:
03
03
2021
pubmed:
12
3
2021
medline:
22
2
2022
entrez:
11
3
2021
Statut:
ppublish
Résumé
Several ribosomal proteins have been shown to adopt for an antimicrobial function as antimicrobial proteins (AMPs). However, information as such is rather limited and their mode of action remains ill-defined. Here we demonstrated that amphioxus RPL30, BjRPL30, was a previously uncharacterized AMP, which was not only capable of binding Gram-negative and Gram-positive bacteria via interaction with LPS, LTA and PGN but also capable of killing the bacteria. We also showed that the residues positioned at 2-46 formed the core region for the antimicrobial activity of BjRPL30. Notably, both the hydrophobic ratio and net charge as well as 3D structures of the residues corresponding to BjRPL30
Identifiants
pubmed: 33705790
pii: S0145-305X(21)00075-6
doi: 10.1016/j.dci.2021.104067
pii:
doi:
Substances chimiques
Recombinant Proteins
0
Ribosomal Proteins
0
ribosomal protein L30
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
104067Informations de copyright
Copyright © 2021 Elsevier Ltd. All rights reserved.