Pre-conditioning of Mesenchymal Stem Cells with Piper longum L. augments osteogenic differentiation.
Alkaline Phosphatase
/ genetics
Cell Differentiation
/ drug effects
Core Binding Factor Alpha 1 Subunit
/ genetics
Gene Expression Regulation, Enzymologic
/ drug effects
Humans
Mesenchymal Stem Cells
/ drug effects
Osteogenesis
/ drug effects
Piper
/ chemistry
Plant Extracts
/ chemistry
RNA, Messenger
/ genetics
Sp7 Transcription Factor
/ genetics
Wharton Jelly
Ayurveda
Mesenchymal Stem Cells
Osteogenic differentiation
Osteoporosis
Journal
Journal of ethnopharmacology
ISSN: 1872-7573
Titre abrégé: J Ethnopharmacol
Pays: Ireland
ID NLM: 7903310
Informations de publication
Date de publication:
12 Jun 2021
12 Jun 2021
Historique:
received:
24
02
2020
revised:
28
11
2020
accepted:
03
03
2021
pubmed:
12
3
2021
medline:
4
11
2021
entrez:
11
3
2021
Statut:
ppublish
Résumé
The Indian Traditional Medicine, Ayurveda prescribes Piper longum L. popularly known as Long Pepper (Pippali) for the treatment of inflammatory and degenerative diseases. Therapeutic benefits of Piper longum L. are mainly attributed to the anti-inflammatory and arthritic potential. This study was aimed to explore the activity of Piper longum L. fruit extract on proliferation and osteogenic differentiation of human Wharton's Jelly Mesenchymal Stem Cells (WJMSCs) to find out it's possible role as anti-osteoporotic agent. Proliferation of WJMSCs treated with Piper longum L. fruit extract was assessed by MTT assay and Cell Cycle Analysis. Effect of Piper longum L. preconditioning on osteogenic differentiation was performed. Ca Significant increase in the proliferation of WJMSCs was observed upon treatment of Piper longum L. at 5 μg/mL (P < 0.001) which can be attributed to the significant decrease in apoptotic cells (P < 0.05) as evidenced by cell cycle analysis. Preconditioning of Piper longum L. (10-100 μg/mL) enhanced Ca We demonstrate for the first time that Piper longum L. fruit extract enhanced osteogenic differentiation of WJMSCs. This finding can be clinically translated into development of an anti-osteoporotic agent.
Identifiants
pubmed: 33705921
pii: S0378-8741(21)00226-9
doi: 10.1016/j.jep.2021.113999
pii:
doi:
Substances chimiques
Core Binding Factor Alpha 1 Subunit
0
Plant Extracts
0
RNA, Messenger
0
RUNX2 protein, human
0
Sp7 Transcription Factor
0
SP7 protein, human
0
Alkaline Phosphatase
EC 3.1.3.1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
113999Informations de copyright
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