SARS-CoV-2 neutralizing human recombinant antibodies selected from pre-pandemic healthy donors binding at RBD-ACE2 interface.
Angiotensin-Converting Enzyme 2
/ chemistry
Animals
Antibodies, Neutralizing
/ genetics
Antibodies, Viral
/ genetics
Antibody Affinity
COVID-19
/ epidemiology
Cell Line
Chlorocebus aethiops
Gene Library
Healthy Volunteers
Host Microbial Interactions
/ immunology
Humans
Immunoglobulin G
/ genetics
Models, Molecular
Mutation
Neutralization Tests
Pandemics
Peptide Library
Protein Interaction Domains and Motifs
Recombinant Proteins
/ genetics
SARS-CoV-2
/ genetics
Spike Glycoprotein, Coronavirus
/ chemistry
Vero Cells
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
11 03 2021
11 03 2021
Historique:
received:
05
06
2020
accepted:
28
01
2021
entrez:
12
3
2021
pubmed:
13
3
2021
medline:
23
3
2021
Statut:
epublish
Résumé
COVID-19 is a severe acute respiratory disease caused by SARS-CoV-2, a new recently emerged sarbecovirus. This virus uses the human ACE2 enzyme as receptor for cell entry, recognizing it with the receptor binding domain (RBD) of the S1 subunit of the viral spike protein. We present the use of phage display to select anti-SARS-CoV-2 spike antibodies from the human naïve antibody gene libraries HAL9/10 and subsequent identification of 309 unique fully human antibodies against S1. 17 antibodies are binding to the RBD, showing inhibition of spike binding to cells expressing ACE2 as scFv-Fc and neutralize active SARS-CoV-2 virus infection of VeroE6 cells. The antibody STE73-2E9 is showing neutralization of active SARS-CoV-2 as IgG and is binding to the ACE2-RBD interface. Thus, universal libraries from healthy human donors offer the advantage that antibodies can be generated quickly and independent from the availability of material from recovering patients in a pandemic situation.
Identifiants
pubmed: 33707427
doi: 10.1038/s41467-021-21609-2
pii: 10.1038/s41467-021-21609-2
pmc: PMC7952403
doi:
Substances chimiques
Antibodies, Neutralizing
0
Antibodies, Viral
0
Immunoglobulin G
0
Peptide Library
0
Recombinant Proteins
0
Spike Glycoprotein, Coronavirus
0
spike protein, SARS-CoV-2
0
ACE2 protein, human
EC 3.4.17.23
Angiotensin-Converting Enzyme 2
EC 3.4.17.23
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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