miR-126 Mimic Counteracts the Increased Secretion of VEGF-A Induced by High Glucose in ARPE-19 Cells.
Adaptor Proteins, Signal Transducing
/ genetics
Cell Line
Class Ia Phosphatidylinositol 3-Kinase
/ genetics
Diabetic Retinopathy
/ genetics
Glucose
/ toxicity
Humans
Hypoxia-Inducible Factor 1, alpha Subunit
/ genetics
MicroRNAs
/ genetics
Oligonucleotides
/ pharmacology
Retinal Neovascularization
/ genetics
Retinal Pigment Epithelium
/ drug effects
Signal Transduction
Up-Regulation
Vascular Endothelial Growth Factor A
/ genetics
Journal
Journal of diabetes research
ISSN: 2314-6753
Titre abrégé: J Diabetes Res
Pays: England
ID NLM: 101605237
Informations de publication
Date de publication:
2021
2021
Historique:
received:
13
11
2020
revised:
25
01
2021
accepted:
03
02
2021
entrez:
12
3
2021
pubmed:
13
3
2021
medline:
11
11
2021
Statut:
epublish
Résumé
Vascular endothelial growth factor-A (VEGF-A) has a pathologic role in microvascular diabetic complication, such as diabetic retinopathy (DR). miR-126 plays an important role in vascular development and angiogenesis by regulating the expression of VEGF-A. Since levels of miR-126 have been found downregulated in diabetes, this study is aimed at investigating whether hyperglycemia affects expression of miR-126 in a retinal pigment epithelium cell line. ARPE-19 cells were transfected with miR-126 inhibitor or with miR-126 mimic and the respective scramble negative control. After 24 hours, medium was replaced and cells were cultured for 24 hours in normal (CTR) or diabetic condition (HG). Then, we analyzed mRNA levels of miR-126, VEGF-A, PI3KR2, and SPRED1. We also evaluated protein amount of HIF-1
Identifiants
pubmed: 33709000
doi: 10.1155/2021/6649222
pmc: PMC7932804
doi:
Substances chimiques
Adaptor Proteins, Signal Transducing
0
HIF1A protein, human
0
Hypoxia-Inducible Factor 1, alpha Subunit
0
MIRN126 microRNA, human
0
MicroRNAs
0
Oligonucleotides
0
SPRED1 protein, human
0
VEGFA protein, human
0
Vascular Endothelial Growth Factor A
0
Class Ia Phosphatidylinositol 3-Kinase
EC 2.7.1.137
Glucose
IY9XDZ35W2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
6649222Informations de copyright
Copyright © 2021 Roberta Sanguineti et al.
Déclaration de conflit d'intérêts
The authors declare that there is no conflict of interest regarding the publication of this paper.
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