An antibody targeting type III secretion system induces broad protection against Salmonella and Shigella infections.
Animals
Antibodies, Bacterial
Antibodies, Monoclonal
/ therapeutic use
Antigens, Bacterial
Dysentery, Bacillary
/ microbiology
Female
HeLa Cells
Humans
Mice
Mice, Inbred BALB C
Salmonella Infections, Animal
/ microbiology
Salmonella typhimurium
/ immunology
Shigella flexneri
/ immunology
Type III Secretion Systems
/ immunology
Journal
PLoS neglected tropical diseases
ISSN: 1935-2735
Titre abrégé: PLoS Negl Trop Dis
Pays: United States
ID NLM: 101291488
Informations de publication
Date de publication:
03 2021
03 2021
Historique:
received:
18
08
2020
accepted:
11
02
2021
revised:
24
03
2021
pubmed:
13
3
2021
medline:
25
6
2021
entrez:
12
3
2021
Statut:
epublish
Résumé
Salmonella and Shigella bacteria are food- and waterborne pathogens that are responsible for enteric infections in humans and are still the major cause of morbidity and mortality in the emerging countries. The existence of multiple Salmonella and Shigella serotypes as well as the emergence of strains resistant to antibiotics requires the development of broadly protective therapies. Recently, the needle tip proteins of the type III secretion system of these bacteria were successfully utilized (SipD for Salmonella and IpaD for Shigella) as vaccine immunogens to provide good prophylactic cross-protection in murine models of infections. From these experiments, we have isolated a cross-protective monoclonal antibody directed against a conserved region of both proteins. Its conformational epitope determined by Deep Mutational Scanning is conserved among needle tip proteins of all pathogenic Shigella species and Salmonella serovars, and are well recognized by this antibody. Our study provides the first in vivo experimental evidence of the importance of this common region in the mechanism of virulence of Salmonella and Shigella and opens the way to the development of cross-protective therapeutic agents.
Identifiants
pubmed: 33711056
doi: 10.1371/journal.pntd.0009231
pii: PNTD-D-20-01486
pmc: PMC7990167
doi:
Substances chimiques
Antibodies, Bacterial
0
Antibodies, Monoclonal
0
Antigens, Bacterial
0
Type III Secretion Systems
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0009231Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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