Development of RT-qPCR assays for the detection and quantification of three carlaviruses infecting hop.

Carlavirus / genetics Humulus Mosaic Viruses Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction
American hop latent virus (AHLV) Carlavirus Detection Hop Hop latent virus (HLV) Hop mosaic virus (HMV) Quantification RT-qPCR

Journal

Journal of virological methods
ISSN: 1879-0984
Titre abrégé: J Virol Methods
Pays: Netherlands
ID NLM: 8005839

Informations de publication

Date de publication:
06 2021
Historique:
received: 10 06 2020
revised: 12 11 2020
accepted: 07 03 2021
pubmed: 13 3 2021
medline: 25 11 2021
entrez: 12 3 2021
Statut: ppublish

Résumé

American hop latent virus (AHLV), hop latent virus (HLV) and hop mosaic virus (HMV) infect members of the Humulus genus worldwide, but very little is known of the biology and etiology of these viruses. A better understanding of these viruses from the molecular level to their economic impact relies on efficient diagnostic assays. Therefore, in this study we developed reverse transcription quantitative polymerase chain reaction (RT-qPCR) assays for the detection of AHLV, HLV, and HMV through an alignment of representative sequences from the National Center for Biotechnology Information (NCBI) database. These assays demonstrated unambiguously their high sensitivity by detecting the respective targets from as low as 10

Identifiants

DOI: 10.1016/j.jviromet.2021.114124 PMID: 33711375
pubmed: 33711375
pii: S0166-0934(21)00063-X
doi: 10.1016/j.jviromet.2021.114124
pii:
doi:

Types de publication

Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

114124

Informations de copyright

Published by Elsevier B.V.

Auteurs

E Beaver-Kanuya (E)

Department of Plant Pathology, Washington State University, Prosser, WA 99350, United States. Electronic address: eunice.kanuya@wsu.edu.

A A Wright (AA)

Department of Plant Pathology, Washington State University, Prosser, WA 99350, United States.

S A Szostek (SA)

Department of Plant Pathology, Washington State University, Prosser, WA 99350, United States.

N Khuu (N)

Department of Plant Pathology, Washington State University, Prosser, WA 99350, United States.

S J Harper (SJ)

Department of Plant Pathology, Washington State University, Prosser, WA 99350, United States.

Articles similaires

Amniotic membrane modulates MMP9 and MMP12 gene and protein expression in experimental model of the hepatic fibrosis.

Ana Paula DA S Alves, Roberta Jenniffer M Teixeira, Raissa M DA Silva et al.
1.00
Animals Matrix Metalloproteinase 12 Matrix Metalloproteinase 9 Amnion Immunohistochemistry

Multiplex PCR assays developed for neglected pathogen detection in undifferentiated acute febrile illness cases in tropical regions.

Leidi Carvajal Aristizabal, Karl Ciuoderis, Laura S Pérez-Restrepo et al.
1.00
Humans Multiplex Polymerase Chain Reaction Sensitivity and Specificity Reproducibility of Results Neglected Diseases

DNMT3A transcriptionally downregulated by KLF5 alleviates LPS-induced inflammatory response and promotes osteogenic differentiation in hPDLSCs.

Jianling Guo, Huijie Jia
1.00
DNA Methyltransferase 3A Lipopolysaccharides Humans Periodontal Ligament Kruppel-Like Transcription Factors

Comparison of DNA extraction procedures for detection of Mycoplasma bovis directly from extended bovine semen straw samples using a commercial M. bovis PCR.

Emma Taylor, Alannah Deeney, Colin Birch et al.
1.00
Animals Cattle Mycoplasma bovis Semen DNA, Bacterial

Classifications MeSH

questionsmedicales.fr › Virus › Virus à ARN › Virus à ARN à brin positif › Flexiviridae › Carlavirus › Development of RT-qPCR assays for the...
questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Rosanae › Rosales › Cannabaceae › Humulus › Development of RT-qPCR assays for the...
questionsmedicales.fr › Virus › Virus des plantes › Virus des mosaïques › Development of RT-qPCR assays for the...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › Réaction de polymérisation en chaîne › Réaction de polymérisation en chaine en temps réel › Development of RT-qPCR assays for the...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › Réaction de polymérisation en chaîne › RT-PCR › Development of RT-qPCR assays for the...