Tityus serrulatus scorpion venom as a potential drug source for Chagas' disease: Trypanocidal and immunomodulatory activity.


Journal

Clinical immunology (Orlando, Fla.)
ISSN: 1521-7035
Titre abrégé: Clin Immunol
Pays: United States
ID NLM: 100883537

Informations de publication

Date de publication:
05 2021
Historique:
received: 19 08 2020
revised: 19 01 2021
accepted: 06 03 2021
pubmed: 13 3 2021
medline: 25 6 2021
entrez: 12 3 2021
Statut: ppublish

Résumé

Current chemical therapies for Chagas Disease (CD) lack ability to clear Trypanosoma cruzi (Tc) parasites and cause severe side effects, making search for new strategies extremely necessary. We evaluated the action of Tityus serrulatus venom (TsV) components during Tc infection. TsV treatment increased nitric oxide and pro-inflammatory cytokine production by Tc-infected macrophages (MØ), decreased intracellular parasite replication and trypomastigotes release, also triggering ERK1/2, JNK1/2 and p38 activation. Ts7 demonstrated the highest anti-Tc activity, inducing high levels of TNF and IL-6 in infected MØ. TsV/Ts7 presented synergistic effect on p38 activation when incubated with Tc antigen. KPP-treatment of MØ also decreased trypomastigotes releasing, partially due to p38 activation. TsV/Ts7-pre-incubation of Tc demonstrated a direct effect on parasite decreasing MØ-trypomastigotes releasing. In vivo KPP-treatment of Tc-infected mice resulted in decreased parasitemia. Summarizing, this study opens perspectives for new bioactive molecules as CD-therapeutic treatment, demonstrating the TsV/Ts7/KPP-trypanocidal and immunomodulatory activity during Tc infection.

Identifiants

pubmed: 33711450
pii: S1521-6616(21)00050-4
doi: 10.1016/j.clim.2021.108713
pii:
doi:

Substances chimiques

Interleukin-6 0
Scorpion Venoms 0
Tumor Necrosis Factors 0
Nitric Oxide 31C4KY9ESH

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

108713

Informations de copyright

Copyright © 2021 Elsevier Inc. All rights reserved.

Auteurs

Pollyana Maria de Oliveira Pimentel (PMO)

Department of Biochemistry and Immunology, Institute of Biological Science, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.

Diego Rodney Rodrigues de Assis (DRR)

Department of Biochemistry and Immunology, Institute of Biological Science, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.

Melisa Gualdrón-Lopez (M)

Department of Biochemistry and Immunology, Institute of Biological Science, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil; ISGlobal, Hospital Clínic - Universitat de Barcelona, Barcelona, Spain.

Andréia Barroso (A)

Department of Biochemistry and Immunology, Institute of Biological Science, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil; Program in Health Sciences: Infectious Diseases and Tropical Medicine/Interdisciplinary Laboratory of Medical Investigation, Faculty of Medicine, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.

Fátima Brant (F)

Department of Biochemistry and Immunology, Institute of Biological Science, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil; Program in Health Sciences: Infectious Diseases and Tropical Medicine/Interdisciplinary Laboratory of Medical Investigation, Faculty of Medicine, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.

Paulo Gaio Leite (PG)

Department of Biochemistry and Immunology, Institute of Biological Science, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.

Bruno Cabral de Lima Oliveira (BC)

Department of Biochemistry and Immunology, Institute of Biological Science, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.

Lisia Esper (L)

Department of Biochemistry and Immunology, Institute of Biological Science, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil; Program in Health Sciences: Infectious Diseases and Tropical Medicine/Interdisciplinary Laboratory of Medical Investigation, Faculty of Medicine, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.

Shaun M K McKinnie (SMK)

Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada; Department of Chemistry and Biochemistry, University of California, Santa Cruz, CA, USA.

John C Vederas (JC)

Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.

Marta do Nascimento Cordeiro (M)

Fundação Ezequiel Dias-FUNED, Minas Gerais, Belo Horizonte, Brazil.

Pablo Victor Mendes Dos Reis (PVM)

Department of Biochemistry and Immunology, Institute of Biological Science, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.

Mauro Martins Teixeira (MM)

Department of Biochemistry and Immunology, Institute of Biological Science, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil; Program in Health Sciences: Infectious Diseases and Tropical Medicine/Interdisciplinary Laboratory of Medical Investigation, Faculty of Medicine, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.

Adriano Monteiro de Castro Pimenta (AM)

Department of Biochemistry and Immunology, Institute of Biological Science, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.

Márcia Helena Borges (MH)

Fundação Ezequiel Dias-FUNED, Minas Gerais, Belo Horizonte, Brazil.

Maria Elena de Lima (ME)

Department of Biochemistry and Immunology, Institute of Biological Science, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil; Santa Casa BH: Instituto de Ensino e Pesquisa, Belo Horizonte, Brazil.

Fabiana Simão Machado (FS)

Department of Biochemistry and Immunology, Institute of Biological Science, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil; Program in Health Sciences: Infectious Diseases and Tropical Medicine/Interdisciplinary Laboratory of Medical Investigation, Faculty of Medicine, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil. Electronic address: machadofs@icb.ufmg.br.

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Classifications MeSH