Survey of diagnostic and typing capacity for enterovirus infection in Italy and identification of two echovirus 30 outbreaks.
Echovirus 30
Enterovirus
Enterovirus molecular typing
Enterovirus survey
Outbreaks
Viral meningitis
Journal
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology
ISSN: 1873-5967
Titre abrégé: J Clin Virol
Pays: Netherlands
ID NLM: 9815671
Informations de publication
Date de publication:
04 2021
04 2021
Historique:
received:
28
08
2020
revised:
12
02
2021
accepted:
14
02
2021
pubmed:
13
3
2021
medline:
18
9
2021
entrez:
12
3
2021
Statut:
ppublish
Résumé
Enterovirus infections can cause a variety of illnesses, ranging from asymptomatic infections to severe illness and death. To support polio eradication activities, in February 2019, the WHO Regional Reference Laboratory for polio in Italy, at the National Institute of Public Health (Istituto Superiore di Sanità), promoted an investigation on non-polio enterovirus laboratory capacity, with the support of the Italian Ministry of Health. The aim was to collect data on the assays used routinely for diagnostic purposes and to characterize enterovirus outbreaks strains by sequence analysis of the Viral Protein 1 region. A questionnaire was administered to public health laboratories through all Italian Regions for 2018 and subsequently, an electronic form for lab-confirmed enterovirus infection reported from February 2019 to January 2020, including patients clinical characteristics, and laboratory data was distributed through 25 laboratories participating the survey. Overall, a homogenous laboratory capacity for enterovirus infection diagnosis was found and 21,000 diagnostic tests were retrospectively reported in 2018. Then, in 2019, two outbreaks of Echovirus 30 were identified and confirmed by molecular analyses. These results underline the need monitor the circulation of non-polio enterovirus to ascertain the real burden of the disease in the country.
Sections du résumé
BACKGROUND
Enterovirus infections can cause a variety of illnesses, ranging from asymptomatic infections to severe illness and death.
AIM
To support polio eradication activities, in February 2019, the WHO Regional Reference Laboratory for polio in Italy, at the National Institute of Public Health (Istituto Superiore di Sanità), promoted an investigation on non-polio enterovirus laboratory capacity, with the support of the Italian Ministry of Health. The aim was to collect data on the assays used routinely for diagnostic purposes and to characterize enterovirus outbreaks strains by sequence analysis of the Viral Protein 1 region.
METHODS
A questionnaire was administered to public health laboratories through all Italian Regions for 2018 and subsequently, an electronic form for lab-confirmed enterovirus infection reported from February 2019 to January 2020, including patients clinical characteristics, and laboratory data was distributed through 25 laboratories participating the survey.
RESULTS
Overall, a homogenous laboratory capacity for enterovirus infection diagnosis was found and 21,000 diagnostic tests were retrospectively reported in 2018. Then, in 2019, two outbreaks of Echovirus 30 were identified and confirmed by molecular analyses.
CONCLUSION
These results underline the need monitor the circulation of non-polio enterovirus to ascertain the real burden of the disease in the country.
Identifiants
pubmed: 33711692
pii: S1386-6532(21)00030-5
doi: 10.1016/j.jcv.2021.104763
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
104763Informations de copyright
Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.