Pyrococcus furiosus Argonaute coupled with modified ligase chain reaction for detection of SARS-CoV-2 and HPV.
Alphapapillomavirus
/ chemistry
Argonaute Proteins
/ chemistry
COVID-19
/ diagnosis
DNA, Viral
/ analysis
Humans
Ligase Chain Reaction
/ methods
Limit of Detection
Mutation
Papillomavirus Infections
/ diagnosis
Pyrococcus furiosus
/ enzymology
RNA, Viral
/ analysis
SARS-CoV-2
/ chemistry
Sensitivity and Specificity
Spike Glycoprotein, Coronavirus
/ genetics
HPV
Ligase chain reaction
Nucleic acid detection
Prokaryotic argonaute
SARS-CoV-2
Journal
Talanta
ISSN: 1873-3573
Titre abrégé: Talanta
Pays: Netherlands
ID NLM: 2984816R
Informations de publication
Date de publication:
15 May 2021
15 May 2021
Historique:
received:
11
11
2020
revised:
17
01
2021
accepted:
24
01
2021
entrez:
14
3
2021
pubmed:
15
3
2021
medline:
24
3
2021
Statut:
ppublish
Résumé
Infectious diseases caused by viruses such as SARS-CoV-2 and HPV have greatly endangered human health. The nucleic acid detection is essential for the early diagnosis of diseases. Here, we propose a method called PLCR (PfAgo coupled with modified Ligase Chain Reaction for nucleic acid detection) which utilizes PfAgo to only use DNA guides longer than 14-mer to specifically cleave DNA and LCR to precisely distinguish single-base mismatch. PLCR can detect DNA or RNA without PCR at attomolar sensitivities, distinguish single base mutation between the genome of wild type SARS-CoV-2 and its mutant spike D614G, effectively distinguish the novel coronavirus from other coronaviruses and finally achieve multiplexed detection in 70 min. Additionally, LCR products can be directly used as DNA guides without additional input guides to simplify primer design. With desirable sensitivity, specificity and simplicity, the method can be extended for detecting other pathogenic microorganisms.
Identifiants
pubmed: 33714462
pii: S0039-9140(21)00075-8
doi: 10.1016/j.talanta.2021.122154
pmc: PMC7875706
pii:
doi:
Substances chimiques
Argonaute Proteins
0
DNA, Viral
0
RNA, Viral
0
Spike Glycoprotein, Coronavirus
0
spike protein, SARS-CoV-2
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
122154Informations de copyright
Copyright © 2021 Elsevier B.V. All rights reserved.
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