A closed-loop optogenetic screen for neurons controlling feeding in Drosophila.
Drosophila
Optogenetics
feeding
neural activation screen
taste
Journal
G3 (Bethesda, Md.)
ISSN: 2160-1836
Titre abrégé: G3 (Bethesda)
Pays: England
ID NLM: 101566598
Informations de publication
Date de publication:
07 05 2021
07 05 2021
Historique:
received:
20
08
2020
accepted:
03
03
2021
pubmed:
15
3
2021
medline:
8
7
2021
entrez:
14
3
2021
Statut:
ppublish
Résumé
Feeding is an essential part of animal life that is greatly impacted by the sense of taste. Although the characterization of taste-detection at the periphery has been extensive, higher order taste and feeding circuits are still being elucidated. Here, we use an automated closed-loop optogenetic activation screen to detect novel taste and feeding neurons in Drosophila melanogaster. Out of 122 Janelia FlyLight Project GAL4 lines preselected based on expression pattern, we identify six lines that acutely promote feeding and 35 lines that inhibit it. As proof of principle, we follow up on R70C07-GAL4, which labels neurons that strongly inhibit feeding. Using split-GAL4 lines to isolate subsets of the R70C07-GAL4 population, we find both appetitive and aversive neurons. Furthermore, we show that R70C07-GAL4 labels putative second-order taste interneurons that contact both sweet and bitter sensory neurons. These results serve as a resource for further functional dissection of fly feeding circuits.
Identifiants
pubmed: 33714999
pii: 6170659
doi: 10.1093/g3journal/jkab073
pmc: PMC8104954
pii:
doi:
Substances chimiques
Drosophila Proteins
0
Banques de données
figshare
['10.25387/g3.14160920']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : CIHR
ID : FDN-148424
Pays : Canada
Informations de copyright
© The Author(s) 2021. Published by Oxford University Press on behalf of Genetics Society of America.
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