Validation and Optimization of Host Immunological Bio-Signatures for a Point-of-Care Test for TB Disease.


Journal

Frontiers in immunology
ISSN: 1664-3224
Titre abrégé: Front Immunol
Pays: Switzerland
ID NLM: 101560960

Informations de publication

Date de publication:
2021
Historique:
received: 18 09 2020
accepted: 18 01 2021
entrez: 15 3 2021
pubmed: 16 3 2021
medline: 6 7 2021
Statut: epublish

Résumé

The development of a non-sputum-based, point-of-care diagnostic test for tuberculosis (TB) is a priority in the global effort to combat this disease, particularly in resource-constrained settings. Previous studies have identified host biomarker signatures which showed potential, but there is a need to validate and refine these for development as a test. We recruited 1,403 adults presenting with symptoms suggestive of pulmonary TB at primary healthcare clinics in six countries from West, East and Southern Africa. Of the study cohort, 326 were diagnosed with TB and 787 with other respiratory diseases, from whom we randomly selected 1005 participants. Using Luminex

Identifiants

pubmed: 33717089
doi: 10.3389/fimmu.2021.607827
pmc: PMC7952865
doi:

Substances chimiques

Biomarkers 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

607827

Subventions

Organisme : FIC NIH HHS
ID : D43 TW009127
Pays : United States
Organisme : Medical Research Council
ID : MC_U190071468
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_UP_A900_1122
Pays : United Kingdom

Informations de copyright

Copyright © 2021 Mutavhatsindi, van der Spuy, Malherbe, Sutherland, Geluk, Mayanja-Kizza, Crampin, Kassa, Howe, Mihret, Sheehama, Nepolo, Günther, Dockrell, Corstjens, Stanley, Walzl, Chegou and the AE-TBC ScreenTB Consortia.

Déclaration de conflit d'intérêts

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Références

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Auteurs

Hygon Mutavhatsindi (H)

Department of Science and Innovation - National Research Foundation (DSI-NRF) Centre of Excellence for Biomedical Tuberculosis Research, South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa.

Gian D van der Spuy (GD)

Department of Science and Innovation - National Research Foundation (DSI-NRF) Centre of Excellence for Biomedical Tuberculosis Research, South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa.

Stephanus T Malherbe (ST)

Department of Science and Innovation - National Research Foundation (DSI-NRF) Centre of Excellence for Biomedical Tuberculosis Research, South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa.

Jayne S Sutherland (JS)

TB Research Group, Medical Research Council Gambia at London School of Hygiene and Tropical Medicine (LSHTM), Banjul, Gambia.

Annemieke Geluk (A)

Department of Infectious Diseases, Leiden University Medical Centre, Leiden, Netherlands.

Harriet Mayanja-Kizza (H)

Department of Internal Medicine and Immunology, School of Medicine, Makerere University, Kampala, Uganda.

Amelia C Crampin (AC)

Karonga Prevention Study, London School of Hygiene and Tropical Medicine, Karonga, Malawi.

Desta Kassa (D)

Infectious and Non-Infectious Diseases Research Directorate, Ethiopian Health and Nutrition Research Institute, Addis Ababa, Ethiopia.

Rawleigh Howe (R)

Department of Immunology, Armauer Hansen Research Institute, Addis Ababa, Ethiopia.

Adane Mihret (A)

Department of Immunology, Armauer Hansen Research Institute, Addis Ababa, Ethiopia.

Jacob A Sheehama (JA)

Department of Biochemistry & Microbiology, School of Medicine, University of Namibia, Windhoek, Namibia.

Emmanuel Nepolo (E)

Department of Biochemistry & Microbiology, School of Medicine, University of Namibia, Windhoek, Namibia.

Gunar Günther (G)

Department of Biochemistry & Microbiology, School of Medicine, University of Namibia, Windhoek, Namibia.

Hazel M Dockrell (HM)

Department of Infection Biology, Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom.

Paul L A M Corstjens (PLAM)

Department of Molecular Cell Biology, Leiden University Medical Centre, Leiden, Netherlands.

Kim Stanley (K)

Department of Science and Innovation - National Research Foundation (DSI-NRF) Centre of Excellence for Biomedical Tuberculosis Research, South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa.

Gerhard Walzl (G)

Department of Science and Innovation - National Research Foundation (DSI-NRF) Centre of Excellence for Biomedical Tuberculosis Research, South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa.

Novel N Chegou (NN)

Department of Science and Innovation - National Research Foundation (DSI-NRF) Centre of Excellence for Biomedical Tuberculosis Research, South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa.

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