Biopolymer-nanotube nerve guidance conduit drug delivery for peripheral nerve regeneration:
Functional recovery
Nerve guidance conduit
Neurotrophic factor
Peripheral nerve regeneration
Sciatic nerve transection
Small-molecule drug delivery
Journal
Bioactive materials
ISSN: 2452-199X
Titre abrégé: Bioact Mater
Pays: China
ID NLM: 101685294
Informations de publication
Date de publication:
Sep 2021
Sep 2021
Historique:
received:
18
01
2021
revised:
12
02
2021
accepted:
12
02
2021
entrez:
15
3
2021
pubmed:
16
3
2021
medline:
16
3
2021
Statut:
epublish
Résumé
Peripheral nerve injuries account for roughly 3% of all trauma patients with over 900,000 repair procedures annually in the US. Of all extremity peripheral nerve injuries, 51% require nerve repair with a transected gap. The current gold-standard treatment for peripheral nerve injuries, autograft repair, has several shortcomings. Engineered constructs are currently only suitable for short gaps or small diameter nerves. Here, we investigate novel nerve guidance conduits with aligned microchannel porosity that deliver sustained-release of neurogenic 4-aminopyridine (4-AP) for peripheral nerve regeneration in a critical-size (15 mm) rat sciatic nerve transection model. The results of functional walking track analysis, morphometric evaluations of myelin development, and histological assessments of various markers confirmed the equivalency of our drug-conduit with autograft controls. Repaired nerves showed formation of thick myelin, presence of S100 and neurofilament markers, and promising functional recovery. The conduit's aligned microchannel architecture may play a vital role in physically guiding axons for distal target reinnervation, while the sustained release of 4-AP may increase nerve conduction, and in turn synaptic neurotransmitter release and upregulation of critical Schwann cell neurotrophic factors. Overall, our nerve construct design facilitates efficient and efficacious peripheral nerve regeneration via a drug delivery system that is feasible for clinical applications.
Identifiants
pubmed: 33718669
doi: 10.1016/j.bioactmat.2021.02.016
pii: S2452-199X(21)00077-3
pmc: PMC7907220
doi:
Types de publication
Journal Article
Langues
eng
Pagination
2881-2893Informations de copyright
© 2021 [The Author/The Authors].
Déclaration de conflit d'intérêts
There is No conflict of interest to publish this research article.
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