Evaluation of fecal immunoassays for canine Echinococcus infection in China.
Animals
Antigens, Protozoan
/ analysis
China
/ epidemiology
Dog Diseases
/ epidemiology
Dogs
Echinococcosis
/ diagnosis
Echinococcus granulosus
/ immunology
Echinococcus multilocularis
/ immunology
Enzyme-Linked Immunosorbent Assay
Feces
/ parasitology
Humans
Reagent Kits, Diagnostic
Sensitivity and Specificity
Tibet
/ epidemiology
Journal
PLoS neglected tropical diseases
ISSN: 1935-2735
Titre abrégé: PLoS Negl Trop Dis
Pays: United States
ID NLM: 101291488
Informations de publication
Date de publication:
03 2021
03 2021
Historique:
received:
05
08
2020
accepted:
17
01
2021
revised:
25
03
2021
pubmed:
16
3
2021
medline:
30
6
2021
entrez:
15
3
2021
Statut:
epublish
Résumé
Human echinococcosis is present worldwide but it is in China that disease prevalence is the highest. In western China, especially in the Tibetan Plateau, the burden of echinococcosis is the most important. Dogs are a major definitive host of Echinococcus and monitoring the presence of Echinococcus worms in dogs is therefore essential to efficiently control the disease. Detection kits based on three different technologies including sandwich ELISA, (indirect) ELISA, and gold immunodiffusion, are currently marketed and used in China. The objective of this work was to assess the efficacy of these kits, in particular with respect to sensitivity and specificity. Four fecal antigen detection kits for canine infection reflecting the three technologies were obtained from companies and tested in parallel on 220 fecal samples. The results indicate that the performance is lower than expected, in particular in terms of sensitivity. The best results were obtained with the sandwich ELISA technology. The gold immunofiltration yielded the poorest results. In all cases, further development is needed to improve the performance of these kits which are key components for the control of echinococcosis.
Identifiants
pubmed: 33720943
doi: 10.1371/journal.pntd.0008690
pii: PNTD-D-20-01404
pmc: PMC7993806
doi:
Substances chimiques
Antigens, Protozoan
0
Reagent Kits, Diagnostic
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0008690Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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